S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Theses (Master's Degree_식품·동물생명공학부)
Enzymes in ginkgolide biosynthetic pathway : cloning and genomic structure
- Issue Date
- 서울대학교 대학원
- 은행(Ginkgo biloba); Ginkgo biloba; cytochrome P450 monooxygenase; Cytochrome p450 monooxygenase; ginkgolide생합성; Ginkgolide biosynthesis; RACE(rapid amplification of cDNA ends); Race(rapid amplification of cdna ends); homology-based PCR; Homology-based pcr; levopimaradiene synthase; Ginkgolide; ginkgolide; Levopimaradiene synthase(lps); 프로모터의 유용인자; Promoter elements; 인트론; Introns; TAIL-PCR(thermal asymmetric interlaced PCR); Tail-pcr(thermal asymmetric interlaced pcr)
- Thesis (master`s)--서울대학교 대학원 :농생명공학부,2004.
- Part 1
The Ginkgo biloba is a perennial woody plant growing throughout the whole region of Korea. It
accumulates various ginkgolides, whose oxidative modification step of terpenoid intermediates in
biosynthesis is monooxygenation by cytochrome P450 monooxygenases. The partial cDNAs from G.
biloba root using homology-based PCR yielded five distinct cytochrome P450 families
(GbCYP∙A, GbCYP∙B, GbCYP∙C, GbCYP∙D, GbCYP∙E). The GbCYP∙E
sequence information derived from rapid amplification of cDNA ends (RACE) was used to produce
1655 bp of a full-length cDNA sequence including 1455 bp of an open reading frame for 485 amino
acids with a deduced size of 55.172 kDa. The deduced amino acid sequence displayed 29% identity
with taxane 14β-hydroxylase of Taxus cuspidata. Also, a cytochrome P450 cDNA which was 2038 bplong
and partially deficient of the 5 -UTR (untranslated region) has been isolated from the
cDNA library of G. biloba root, naming it as GbCYP∙F. This showed to have an open reading
frame 1761 bp-long and a 586 amino acid sequence with its size 66.196 kDa. The deduced amino
acid sequences of GbCYP∙F displayed 75% identity with CYP97B2 monooxygenase of Glycine max.
This is the first report on cDNA isolation of the cytochrome P450 genes from G. biloba.
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