Characterization of two cucumber green mottle mosaic virus strains isolated in Korea

Abstract

In Korea, CGMMV was first reported in 1989 and caused widespread Blood flesh in watermelon field with considerable economic losses in 1995. Since then, viral disease on cucurbit plants in many fields are being reported occasionally. The complete nucleotide sequences of the genomic RNAs of Cucumber green mottle mosaic virus Korean oriental melon strain (CGMMV-KOM) and Korean watermelon strain (CGMMV-KW) have been determined and small size RNAs in virion of two CGMMV isolates were characterized as the subgenomic RNAs. Each CGMMV strain consists of 6,424 nucleotides of plus-strand RNA genome. The CGMMV genome consists of 60 and 176 nucleotides of 5' and 3' non-translated regions (NTRs), respectively, and contains four putative open reading frames (ORF1-4). ORFs 1 to 4 encode for proteins of 129, 186, 29, and 17.4-kDa, respectively. Even though two isolates of CGMMV, CGMMV-KOM and CGMMV-KW have similarity over 98.3%, they showed different symptom on the inoculum leaves of Chenopodium amaranticolor; CGMMV-KOM expressed no symptom but CGMMV-KW expressed chlorotic ringspot.