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Bioimaging of the unbalanced expression of microRNA9 and microRNA9* during the neuronal differentiation of P19 cells
Cited 49 time in
Web of Science
Cited 50 time in Scopus
- Authors
- Issue Date
- 2008-04-16
- Publisher
- Wiley-Blackwell
- Citation
- FEBS Journal 275(10), 2605-2516
- Keywords
- Animals ; Base Sequence ; Cell Differentiation/*physiology ; Cell Line, Tumor ; Genes, Reporter ; Mice ; Mice, Nude ; MicroRNAs/genetics/*metabolism ; Molecular Sequence Data ; Neurons/cytology/*physiology ; Brain/cytology/embryology ; Gene Expression Regulation, Developmental
- Abstract
- Generally, the 3'-end of the duplex microRNA (miR) precursor (pre-miR) is known to be stable in vivo and serve as a mature form of miR. However, both the 3'-end (miR9) and 5'-end (miR9*) of a brain-specific miR9 have been shown to function biologically in brain development. In this study, real-time PCR analysis and in vitro/in vivo bioluminescent imaging demonstrated that the upstream region of a primary miR9-1 (pri-miR9-1) can be used to monitor the highly expressed pattern of endogenous pri-miR9-1 during neurogenesis, and that the Luciferase reporter gene can image the unequal expression patterns of miR9 and miR9* seen during the neuronal differentiation of P19 cells. This demonstrates that our bioimaging system can be used to study the participation of miRs in the regulation of neuronal differentiation.
- ISSN
- 1742-464X (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18410378
https://hdl.handle.net/10371/67794
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