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Bioimaging of the unbalanced expression of microRNA9 and microRNA9* during the neuronal differentiation of P19 cells

Cited 48 time in Web of Science Cited 48 time in Scopus
Authors
Ko, Mee Hyang; Kim, Soonhag; Hwang, Do Won; Ko, Hae Young; Kim, Young Ha; Lee, Dong Soo
Issue Date
2008-04-16
Publisher
Wiley-Blackwell
Citation
FEBS Journal 275(10), 2605-2516
Keywords
AnimalsBase Sequence*Brain/cytology/embryologyCell Differentiation/*physiologyCell Line, Tumor*Gene Expression Regulation, DevelopmentalGenes, ReporterMiceMice, NudeMicroRNAs/genetics/*metabolismMolecular Sequence DataNeurons/cytology/*physiology
Abstract
Generally, the 3'-end of the duplex microRNA (miR) precursor (pre-miR) is known to be stable in vivo and serve as a mature form of miR. However, both the 3'-end (miR9) and 5'-end (miR9*) of a brain-specific miR9 have been shown to function biologically in brain development. In this study, real-time PCR analysis and in vitro/in vivo bioluminescent imaging demonstrated that the upstream region of a primary miR9-1 (pri-miR9-1) can be used to monitor the highly expressed pattern of endogenous pri-miR9-1 during neurogenesis, and that the Luciferase reporter gene can image the unequal expression patterns of miR9 and miR9* seen during the neuronal differentiation of P19 cells. This demonstrates that our bioimaging system can be used to study the participation of miRs in the regulation of neuronal differentiation.
ISSN
1742-464X (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18410378

http://hdl.handle.net/10371/67794
DOI
https://doi.org/10.1111/j.1742-4658.2008.06408.x
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College of Medicine/School of Medicine (의과대학/대학원)Nuclear Medicine (핵의학전공)Journal Papers (저널논문_핵의학전공)
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