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Inhibitory effects of triphlorethol-A on MMP-1 induced by oxidative stress in human keratinocytes via ERK and AP-1 inhibition
Cited 22 time in
Web of Science
Cited 20 time in Scopus
- Authors
- Issue Date
- 2008-06-24
- Publisher
- Taylor & Francis
- Citation
- J Toxicol Environ Health A. 71(15):992-999
- Keywords
- Blotting, Western ; Catalase/metabolism ; Cell Survival/drug effects ; Cells, Cultured ; Comet Assay ; DNA/drug effects ; DNA Damage ; Enzyme Induction ; Extracellular Signal-Regulated MAP Kinases/*antagonists & inhibitors ; Fluorescent Antibody Technique, Indirect ; Free Radical Scavengers/*pharmacology ; Humans ; Hydrogen Peroxide/pharmacology ; Keratinocytes/*drug effects/enzymology ; Matrix Metalloproteinase 1 ; Oxidants/pharmacology ; Oxidative Stress ; Phloroglucinol/*analogs & derivatives/pharmacology ; Transcription Factor AP-1/*antagonists & inhibitors
- Abstract
- Oxidative stress is known to generate reactive oxygen species (ROS) in cells, which subsequently induce the synthesis of matrix metalloproteinases (MMP) and an aging phenomenon. The protective effects of triphlorethol-A, derived from Ecklonia cava, were investigated against hydrogen peroxide (H(2)O(2))-induced damage using human skin keratinocytes. Data showed that triphlorethol-A inhibited ROS formation, induced catalase expression, inhibited DNA damage, and increased cell viability in keratinocytes. Triphlorethol-A treatment significantly reduced MMP-1 expression and production, compared to H(2)O(2)-treated cells. In addition, triphlorethol-A abrogated the activation of extracellular signal regulated protein kinase (ERK), which originates upstream of MMP-1 expression, and was induced by H(2)O(2) treatment. Moreover, triphlorethol-A inhibited DNA binding activity of activator protein-1 (AP-1), a downstream transcription factor of ERK. Data indicate that the antioxidative properties of triphlorethol-A involve the inhibition of MMP-1 via ERK and AP-1 inhibition.
- ISSN
- 1528-7394 (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18569608
http://pdfserve.informaworld.com/271715_758494928_794223963.pdf
https://hdl.handle.net/10371/67886
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