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Differentiation of Mycobacterium species by analysis of the heat-shock protein 65 gene (hsp65)

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dc.contributor.authorKim, Hong-
dc.contributor.authorKim, Sun-Hyun-
dc.contributor.authorShim, Tae-Sun-
dc.contributor.authorKim, Mi-na-
dc.contributor.authorBai, Gill-Han-
dc.contributor.authorPark, Young-Gil-
dc.contributor.authorLee, Sueng-Hyun-
dc.contributor.authorChae, Gue-Tae-
dc.contributor.authorCha, Chang-Yong-
dc.contributor.authorKook, Yoon-Hoh-
dc.contributor.authorKim, Bum-Joon-
dc.date.accessioned2010-06-28T03:27:10Z-
dc.date.available2010-06-28T03:27:10Z-
dc.date.issued2005-07-15-
dc.identifier.citationInt J Syst Evol Microbiol. 55(Pt 4), 1649-1656en
dc.identifier.issn1466-5026 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16014496-
dc.identifier.urihttps://hdl.handle.net/10371/67895-
dc.description.abstractThe nucleotide sequences (604 bp) of partial heat-shock protein genes (hsp65) from 161 Mycobacterium strains containing 56 reference Mycobacterium species and 105 clinical isolates were determined and compared. hsp65 sequence analysis showed a higher degree of divergence between Mycobacterium species than did 16S rRNA gene analysis. Generally, the topology of the phylogenetic tree based on the hsp65 DNA sequences was similar to that of the 16S rRNA gene, thus revealing natural relationships among Mycobacterium species. When a direct sequencing protocol targeting 422 bp sequences was applied to 70 non-tuberculous mycobacterium (NTM) clinical isolates, all NTMs were clearly identified. In addition, an XhoI PCR restriction fragment length polymorphism analysis method for the differentiation of Mycobacterium tuberculosis complex from NTM strains was developed during this study. The results obtained suggest that 604 bp hsp65 sequences are useful for the phylogenetic analysis and species identification of mycobacteria.en
dc.language.isoenen
dc.publisherSociety for General Microbiologyen
dc.subjectBacterial Proteins/*geneticsen
dc.subject*Bacterial Typing Techniquesen
dc.subjectChaperonins/*geneticsen
dc.subjectDNA, Bacterial/analysisen
dc.subjectDNA, Ribosomal/analysisen
dc.subjectDeoxyribonucleases, Type II Site-Specific/metabolismen
dc.subjectHumansen
dc.subjectMolecular Sequence Dataen
dc.subjectMycobacterium/*classification/geneticsen
dc.subjectMycobacterium tuberculosis/classification/geneticsen
dc.subjectPhylogenyen
dc.subjectPolymerase Chain Reactionen
dc.subjectPolymorphism, Restriction Fragment Lengthen
dc.subjectRNA, Ribosomal, 16S/geneticsen
dc.subjectSequence Analysis, DNAen
dc.subjectSpecies Specificityen
dc.titleDifferentiation of Mycobacterium species by analysis of the heat-shock protein 65 gene (hsp65)en
dc.typeArticleen
dc.contributor.AlternativeAuthor김홍-
dc.contributor.AlternativeAuthor김선현-
dc.contributor.AlternativeAuthor심태선-
dc.contributor.AlternativeAuthor김미나-
dc.contributor.AlternativeAuthor배길한-
dc.contributor.AlternativeAuthor박영길-
dc.contributor.AlternativeAuthor이승현-
dc.contributor.AlternativeAuthor채규태-
dc.contributor.AlternativeAuthor차창용-
dc.contributor.AlternativeAuthor국윤호-
dc.contributor.AlternativeAuthor김범준-
dc.identifier.doi10.1099/ijs.0.63553-0-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Microbiology (미생물학전공)Journal Papers (저널논문_미생물학전공)
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