S-Space College of Medicine/School of Medicine (의과대학/대학원) Obstetrics & Gynecology (산부인과전공) Journal Papers (저널논문_산부인과학전공)
Identification of proteomic biomarkers of preeclampsia in amniotic fluid using SELDI-TOF mass spectrometry
- Park, Joong Shin; Oh, Kyoung-Jin; Norwitz, Errol R; Han, Joong-Soo; Choi, Hye-Jin; Seong, Hyo Suk; Kang, Yoon Dan; Park, Chan-Wook; Kim, Byoung Jae; Jun, Jong Kwan; Syn, Hee Chul
- Issue Date
- SAGE Publications
- Reprod Sci. 2008;15(5):457-468
- Adult; Amniotic Fluid/*chemistry/metabolism; Apolipoprotein A-I/*analysis; Biological Markers/analysis; Diagnosis, Differential; Female; Humans; Hypertension/diagnosis/metabolism; Infant, Newborn; Pilot Projects; Pre-Eclampsia/diagnosis/*metabolism; Pregnancy; Proteomics/*methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
- OBJECTIVE: To identify proteomic biomarkers in amniotic fluid (AF) that can distinguish preeclampsia (PE) from chronic hypertension (CHTN) and normotensive controls (CTR). METHODS: AF from women with PE, CHTN, and CTR were subjected to proteomic analysis by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. RESULTS: Proteomic profiling of AF identified 2 biomarkers: peak X (17399.11 Da), which distinguished PE from CTR, and peak Y (28023.34 Da), which distinguished PE and CHTN from CTR. High performance liquid chromatography fractions containing the biomarkers were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and in-gel tryptic digestion. The biomarkers were matched to proapolipoprotein A-I (peak Y) and a functionally obscure peptide, SBBI42 (peak X). Western blot analysis confirmed that AF from PE and CHTN had higher proapolipoprotein A-I levels than CTR. CONCLUSION: Proteomic analysis of AF can distinguish PE from CHTN and CTR. The discriminatory proteins were identified as proapolipoprotein A-I and SBBI42.
- 1933-7205 (Electronic)
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