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Identification of proteomic biomarkers of preeclampsia in amniotic fluid using SELDI-TOF mass spectrometry

Cited 41 time in Web of Science Cited 42 time in Scopus
Authors
Park, Joong Shin; Oh, Kyoung-Jin; Norwitz, Errol R; Han, Joong-Soo; Choi, Hye-Jin; Seong, Hyo Suk; Kang, Yoon Dan; Park, Chan-Wook; Kim, Byoung Jae; Jun, Jong Kwan; Syn, Hee Chul
Issue Date
2008-06-27
Publisher
SAGE Publications
Citation
Reprod Sci. 2008;15(5):457-468
Keywords
AdultAmniotic Fluid/*chemistry/metabolismApolipoprotein A-I/*analysisBiological Markers/analysisDiagnosis, DifferentialFemaleHumansHypertension/diagnosis/metabolismInfant, NewbornPilot ProjectsPre-Eclampsia/diagnosis/*metabolismPregnancyProteomics/*methodsSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
Abstract
OBJECTIVE: To identify proteomic biomarkers in amniotic fluid (AF) that can distinguish preeclampsia (PE) from chronic hypertension (CHTN) and normotensive controls (CTR). METHODS: AF from women with PE, CHTN, and CTR were subjected to proteomic analysis by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. RESULTS: Proteomic profiling of AF identified 2 biomarkers: peak X (17399.11 Da), which distinguished PE from CTR, and peak Y (28023.34 Da), which distinguished PE and CHTN from CTR. High performance liquid chromatography fractions containing the biomarkers were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and in-gel tryptic digestion. The biomarkers were matched to proapolipoprotein A-I (peak Y) and a functionally obscure peptide, SBBI42 (peak X). Western blot analysis confirmed that AF from PE and CHTN had higher proapolipoprotein A-I levels than CTR. CONCLUSION: Proteomic analysis of AF can distinguish PE from CHTN and CTR. The discriminatory proteins were identified as proapolipoprotein A-I and SBBI42.
ISSN
1933-7205 (Electronic)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18579854

http://rsx.sagepub.com/cgi/reprint/15/5/457.pdf

http://hdl.handle.net/10371/68095
DOI
https://doi.org/10.1177/1933719108316909
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College of Medicine/School of Medicine (의과대학/대학원)Obstetrics & Gynecology (산부인과전공)Journal Papers (저널논문_산부인과학전공)
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