S-Space College of Medicine/School of Medicine (의과대학/대학원) Ophthalmology (안과학전공) Journal Papers (저널논문_안과학전공)
Susceptibility of porcine keratocytes to immune-mediated damage in xeno-related rejection
- Kim, M K; Oh, J Y; Lee, H I; Ko, J H; Lee, H J; Lee, J H; Wee, W R
- Issue Date
- Transplant Proc. 40(2):564-569
- Animals; Antibody-Dependent Cell Cytotoxicity; Cells, Cultured; Cornea/*cytology/*immunology; Corneal Transplantation/*immunology; DNA Primers; Endothelial Cells/*cytology/*immunology; Endothelins/genetics; Graft Rejection/*immunology/pathology; Humans; Reverse Transcriptase Polymerase Chain Reaction; Swine; Transplantation, Heterologous/*immunology/pathology; Vimentin/genetics
- We admixed cultured porcine keratocytes or corneal endothelial cells in the presence of human sera or peripheral blood mononuclear cells (PBMCs) for 4 to 72 hours to investigate their immune-related susceptibilities to xeno-related rejection. We evaluated complement deposition at 48 hours by flow cytometry after staining with the C3 anti-goat cy3 antibody. The inhibition of proliferation of porcine corneal cells by human sera was examined using the 3-[4,5-dimethy/thiazol-2,5-dephenyl tetrazolium bromide (MTT) assay over 24 to 72 hours. The amount of 51chromium (Cr)-release was estimated after a reaction between the porcine cells and human PBMCs for 4 hours. There was greater C3 deposition in keratocytes (60.2%) than in endothelial cells (26.9%; P = .05, Mann-Whitney U test). Both keratocytes and endothelial cells showed significant levels of proliferative inhibition over a period of 72 hours. The number of 51Cr-release cells on interleukin-2 addition was significantly higher among keratocytes (88.0%) than endothelial cells (51.4%) at a 1:100 target:effector ratio (P = .04, Mann-Whitney U test). Our present data suggested that porcine keratocytes might be key target cells in xeno-related rejections when the porcine cornea is transplanted to primates.
- 0041-1345 (Print)
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