S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Theses (Master's Degree_식품·동물생명공학부)
Isolation and identification of antibiotics from endophytic fungi
식물 내생 진균에서 항생물질 분리 및 동정
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- 서울대학교 대학원
- Endophytic fungi; Alternariol monomethyl ether; Antibiotics; Alternaria spp.; Penicillium spp.
- Thesis (master`s)--서울대학교 대학원 :농생명공학부,2003.
- Endophytic fungi, found within the living tissues of the plant, are microbes that seem to have
established a relationship with a higher plant. A number of bioactive metabolites have been
found from those endophytic fungi. The aim of this study was to find antibiotics from endophytic
fungi. Endophytic fungi were isolated from tissue of trees to screen activity against human and
plant pathogens (Pythium ultimum, Candida albicans, Magnaporthe grisea). Forty-one fungi had
activity against P. ultimum, thirty-six against C. albicans and fifty-five against the formation
of appressorium of M. grisea. Bioactive metabolites from fungi, which showed activity against P.
ultimum and M. grisea, were extracted with CH2Cl2 after 20 days old fermentation broth. Three
compounds were isolated through TLC and HPLC. Their strucutures characterized through NMR, IR
and mass spectrometries. Alternariol monomethyl ether (Compound 3), which was produced by
Alternaria spp. isolated from Acacia mungium leaf, was isolated. The compound 1, which was
produced by Penicillium spp. from Eucalyptus hybrid, was 1, 3-Dihydroxy-9-methyl-8, 9, 10, 11,
12, 13-hexahydro-5H, 7H-7, 8-diaza- benzocyclodecene-6, 14-dione. The compound2, which was
produced by Alternaria spp. from Acacia mungium leaf, was 7-(7-Hydroxy-oct-2-enoyl)-2-
oxa-bicyclo3.2.0hepta-1(7),3, 5-trinen-3- yl]-acetic acid.
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