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Characterization of Xanthomonas axonopodis pv. glycines plasmids : Xanthomonas axonopodis pv. glycines에 존재하는 플라스미드의 특성화

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Authors

최세정

Advisor
황인규
Issue Date
2003
Publisher
서울대학교 대학원
Keywords
비병원성유전자Avirulence geneconjugative 플라스미드Conjugative plasmidXanthomonas axonopodis pv. glycines(콩불마름병원균)MobilizationXanthomonas axonopodis pv. glycines
Description
Thesis (master`s)--서울대학교 대학원 :농생명공학부,2003.
Abstract
Xanthomonas axonopodis pv. glycines causes bacterial pustules on soybean. We have isolated
plasmids pAG1 and pXAG81 of Xanthomonas axonopodis pv. glycines isolate AG1 and 8ra,
respectively, and sequenced two plasmids. The size of plasmids, pAG1 and pXAG81, was 15,149 base
pairs (bp) and 26,727 bp, respectively. Fifteen possible open reading frames (ORFs) are present
in pAG1 and twenty six possible ORFs are present in pXAG81. The pAG1 encoded genes homologous to
avrBs3, tnpA, tnpR, repA, htrA, three parAs, M.XmaI, R.XmaI, and six hypothetical proteins. G+C
content of pAG1 was 62.40%. The pXAG81 encoded genes homologous to avrBs3, tnpA, tnpR, repA,
htrA, two parAs, pemI, pemK, mobA, mobB, mobC, mobD, mobE, trwB, traF, traH, ISxac2, and eleven
hypothetical proteins. G+C content of pXAG81 was 61.44%. Two hundred twenty five isolates were
obtained from the soybean bacterial pustules found in Korea during 2000-2001. Chromosomal and
plasmid DNA were hybridized with DNA fragment carrying avrBs3 or tra gene homolog. The numbers
of avr gene varied from 3 in AG1 to 8 in AG166. Two hundred seventeen isolates appeared to carry
conjugative plasmids, and thirty eight isolates appeared to carry non-conjugative plamids. This
indicated that conjugative plamids are dominant among isolates of X. axonopodis pv. glycines
during 2000-2001 in Korea.
Language
English
URI
http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000057850

https://hdl.handle.net/10371/68515
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