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Development of probiotic Lactobacillus producing hGH fused with Fc fragment of IgG

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Authors
이창훈
Advisor
최윤재
Issue Date
2004
Publisher
서울대학교 대학원
Keywords
유산균The MHC class-I related IgG receptor성장호르몬FcRn면역글로블린GLactobacillus. brevisFcRnhuman IgGHuman growth hormoneTranscytosis
Description
Thesis(master`s)--서울대학교 대학원 :농생명공학부 동물자원과학과,2004.
Abstract
It has been understood that the MHC class-I related IgG receptor has mutiple functions such as
the passive delivery of immunoglobulin (Ig)Gs from mother to young and the regulation of serum
IgG levels. In addition, FcRn expression in tissues such as liver, mammary gland, and adult
intestine suggests that it may modulate IgG transport at these sites. These diverse functions
are apparently brought about by the ability of FcRn to bind IgGs and transport them within and
across cells. Futhermore, it is well understood that the Fc amino acids are highly conserved in
rodents and man and interact with residues primarily located on the alpha2 domain of FcRn and
rodent FcRn has affinity to human IgG.
As information about the MHC class-I related IgG receptor is revealed more and more, people
have interested in developing the Fc fragment of IgG-based therapeutics. Therefore we produced
the lactobacillus producing a fusion protein , consisting of the mature part of hGH and Fc
fragment of human IgG1 fused to the secretional signal of the S-layer protein of Lactobacillus.
brevis. Because Lactobacillus has capability to colonize on the mammalian intestine and to
secret the human GH coupled human Fc , the lactobacillus producing the human GH coupled Fc
could deliver the fusion protein across intestinal barrier to the blood stream. The expressed
fusion protein was analysed by western-blotting and ELISA method. To show that the hGH-Fc
fusion protein have transcytosis activity of IgG and growth promoting activity of hGH, We tested
the biological activities of the fusion protein through in vitro transcytosis assay and in vivo
test. As a result, lactobacillus to producing the fusion protein , hGH-Fc showed that Lb.brevis
could efficiently secrete biological active candidate fusion protein and that the fusion protein
have two functional activities of hGH and transcytosis.
Language
English
URI
http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000056682

http://hdl.handle.net/10371/68516
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Theses (Master's Degree_식품·동물생명공학부)
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