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Isolation and characterization of (RS)-2-(4-Chloro-2-methylphenoxy)propionic acid-degrading bacteria from soils
토양에서 제초제 MCPP 분해 세균의 분리 및 생리유전학적 특성에 대한 연구

DC Field Value Language
dc.contributor.advisor가종억-
dc.contributor.author임종성-
dc.date.accessioned2010-07-08T23:08:06Z-
dc.date.available2010-07-08T23:08:06Z-
dc.date.copyright2003.-
dc.date.issued2003-
dc.identifier.urihttp://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000057721-
dc.identifier.urihttp://hdl.handle.net/10371/68521-
dc.descriptionThesis (master`s)--서울대학교 대학원 :농생명공학부,2003.en
dc.description.abstractFifteen numerically dominant (RS)-2-(4-Chloro-2-methylphenoxy)propionic acid ( MCPP,
mecoprop ) degrading bacteria were isolated from soils, and their genetic and phenotypic
characteristics were investigated. The mecoprop-degrading microorganisms were ubiquitous in golf
course soils, while no positive degradation was observed from agricultural soils. The isolate
were able to utilize the herbicide MCPP as a sole source of carbon and energy. Nine out of the
fifteen isolates were able to degrade both (R)-mecoprop and (S)-mecoprop, five strains ( MP1, 4,
9, 18, 24 ) could degrade only (S)-mecoprop and one strain exhibited enantioselective
degradation of (R)-mecoprop. The isolates also vigorously utilized other phenoxyacid herbidides,
including 2,4-D, 2,4-DP, and MCPA, as sole carbon sources. Analysis of 16S rDNA sequences
indicated that the all isolates were related to members of the genus, Sphingomonas. The REP-PCR
analysis of the isolates revealed that some strains isolated from different soils exhibited
identical REP-PCR patterns. All of the isolates were observed to have plasmid DNAs. Isolates,
MP4, MP17, and MP24 identified as the same strains by 16s rDNA sequence and REP-PCR analyses
exhibited different plasmid profile and degradation properties to each other.
en
dc.format.extentvi, 52 leavesen
dc.language.isoenen
dc.publisher서울대학교 대학원en
dc.subject제초제en
dc.subject(RS)-2-(4-Chloro-2-methylphenoxy)propionic aciden
dc.subjectMCPPen
dc.subjectHerbicideen
dc.subject플라스미드en
dc.subjectBacteriaen
dc.subject16S rDNAen
dc.subjectEnantioselective degradationen
dc.subjectREP-PCRen
dc.subject분해양상en
dc.titleIsolation and characterization of (RS)-2-(4-Chloro-2-methylphenoxy)propionic acid-degrading bacteria from soilsen
dc.title.alternative토양에서 제초제 MCPP 분해 세균의 분리 및 생리유전학적 특성에 대한 연구en
dc.typeThesisen
dc.contributor.department농생명공학부-
dc.description.degreeMasteren
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Theses (Master's Degree_식품·동물생명공학부)
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