S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Theses (Master's Degree_식품·동물생명공학부)
Profiling changes in gene expression during proliferation, differentiation, and apoptosis of human mammary epithelial cells using cDNA microarray
cDNA microarrays를 이용하여 유선 상피세포의 성장·분화·퇴화과정에서의 gene expression profiling 변화에 관한 연구
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- 서울대학교 대학원
- Thesis (master`s)--서울대학교 대학원 :농생명공학부,2003.
- The mammary gland undergoes striking changes in morphology and function during development and
puberty and adult life. During each menstrual cycle, and particularly during pregnancy, mammary
epithelial cells undergo cycles of proliferation, differentiation, and apoptotic cell death.
During pregnancy, mammary epithelial cells proliferate and form lobulo-alveolar structures that
prepare the gland for lactation (Traurig, H. H., 1967; Borst, D. W. et al., 1982; Zwierzchowski,
L. et al., 1984). At late pregnancy, prior to parturition, breast epithelial cells cease
proliferation and functionally differentiate into cells that express and secrete milk proteins.
Throughout lactation, epithelial cells remain quiescent but continue to express milk proteins
(Traurig, H. H., 1967). After weaning, the mammary gland undergoes involution, a phase of
extensive remodeling characterized by degradation of ECM and epithelial cell death by apoptosis.
The molecular mechanisms that regulate and coordinate the changes in mammary epithelial cell
phenotypes are poorly understood. Genes that are up- or down-regulated at different
developmental stages have been identified, but little is known about how they are regulated and
In this study, we made a novel cell culture model for studying proliferation, differentiation
and apoptosis in the human mammary epithelial cells. Proliferation, differentiation, apoptosis
of human mammary epithelial cells was induced by using specific hormones, starvation, and
apoptosis inducer. To identify physiologically specific gene expression patterns in human mammry
epithelial cells was used cDNA microarrays.
Analysis of gene expression changes at the RNA level using cDNA against an array of 8286 human
genes showed that 7935 (95%) of genes were expressed in the HMEC. A total of 388 genes (4.6%)
were found to be regulated during HMEC differentiaton. A total of 411genes (4.9%) were found to
be regulated during HMEC apoptosis by serum-free medium. A total of 119 genes (1.4%) were found
to be regulated during HMEC apoptosis by anti-Fas antibodies. We were performed by
bioinformatics program, to search for the functions of genes identified by cDNA microarrays.
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