S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Theses (Master's Degree_식품·동물생명공학부)
Singlet oxygen reaction of mycosporine glycine as a suppressor of photosensitization in marine organisms
해양 생물체에서 광증감반응의 저해제로서 Mycosporine glycine과 단일항산소와의 반응
- Issue Date
- 서울대학교 대학원
- 항산화제; Antioxidant; 광증감적 피해; Mycosporine-like amino acids; 광보호; Mycosporine glycine; 단일항산소; Photosensitized damage; Quenching 반응; Photoprotection; Singlet oxygen quenching reaction
- Thesis (master`s)--서울대학교 대학원 :농생명공학부,2003.
- This report concerns physiological function of mycosporine-like amino acids (MAA) as an active
defense against photooxidative sunlight effects in marine organisms. In biological model systems,
mycosporine glycine (MG) that is a representative member of MAA family was found to efficiently
suppress singlet oxygen (1O2)-mediated photodynamic effects, such as hemolysis of erythrocytes,
inhibition of mitochondrial respiratory activity and inactivation of lactate dehydrogenase. The
relative singlet oxygen (1O2) quenching efficiency was assessed by ESR experiment with a 1O2
trap compound 2,2,6,6-tetramethyl-4-piperidone. Since MG turned out to be more effective than a
well known scavenger histidine, the rate constant of its reaction with 1O2 was measured by both
steady state method and time resolved method, the former being based on inhibition of the selfsensitized
photooxidation of rubrene and the latter on increase in the decay rate constant of
1O2 measured from time-resolved decay signal of 1O2 phosphorescence by the added MG. The rate
constant of MG-1O2 reaction was determined to be 6.5 x 107 M-1s-1 by the rubrene method and 5.6
x 107 M-1s-1 by the time-resolved method. The results suggest that MG probably together with
some other active MAA may play an important role in protecting marine organisms against
sunlight-damage by eliminating 1O2 generated from certain endogenous photosensitizers.
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