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한국에서의 선인장 줄기곰팡이병 : 새로운 병원균과 줄기썩음병균 Bipolaris cactivora의 잠재적 분화형
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 김영호 | - |
dc.contributor.author | 김정호 | - |
dc.date.accessioned | 2010-07-08T23:32:55Z | - |
dc.date.available | 2010-07-08T23:32:55Z | - |
dc.date.copyright | 2003. | - |
dc.date.issued | 2003 | - |
dc.identifier.uri | http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000057167 | - |
dc.identifier.uri | https://hdl.handle.net/10371/68541 | - |
dc.description | Thesis (master`s)--서울대학교 대학원 :농생명공학부,2003. | en |
dc.description.abstract | Cacti are exotic plants in Korea, and cultivated mainly as indoor
ornamental plants. Graft cacti, which are composed of two different species of cylindrical cactus as stock and globular cactus as scion, are economically important for exporting and domestic uses in Korea. For production of graft cacti, the main problems are diseases are caused by fungi. In 1999 and 2000, cactus stem diseases caused by fungi were surveyed in greenhouses at Suwon, Anseong, Eumseong, Yeoju, Goyang, Cheoan, and Gimcheon in Korea. Form stem rot or spot symptoms, fungi were isolated, among which 116 Alternaria spp., 82 Fusarium spp., 62 Bipolaris cactivora, 8 Colletotrichum sp., and many unknown fungi, Colletotrichum species was identified as C. gloeosporioides (Teleomorph Glomerella cingulata) based on cultural and morphological characteristics of the fungus. It caused the typical Colletrichum stem rot disease by artificial inoculation, proving its pathogenicity. However, two cactus species, Myrtillocactus geomentrizans and Cereus peruvianus appeared to be resistant to the pathogen, which may be used as resistant sources. Two Alternaria species, A. alternata and A. tenuissima, were identified based on their mycological characteristics. In inoculation test, A. tenuissima showed more virulence to cactus plants than A. alternata. A. alternata induced the same spots as natural ones, and believe to be the causal agent of the disease. A. alternata may be opportunistically involved in cactus stem rotting as a secondary invader. Fungal morphology and random amplified polymorphic DNA (RAPD) analysis of B. cactivora were examined. Colony morphology was differentiated into four types (A, B, D, and D), of which types A, C and D had conidial type Ⅰ(more conical at spore end) and type B, conidial type Ⅱ (more round). The conidial morphology was quantitatively analyzed using image analysis. Twenty-eight amplified fragments were produced by polymerase chain reaction (PCR) with a set of two random primers, and the sizes of amplified DNA fragments ranged approximately from 0.1 to 2.3 kb. The 62 isolates of B. cactivora were classified into two major genomic DNA RAPD groups at the genomic similarity of 97.7% and 95.1%, respectively. Cluster analysis of genetic similarity among the isolates generated the dendrogram that clearly separated all isolates into Ⅰ or Ⅱ conidial types. However, no or little difference was found in pathogenicity between conidial type Ⅰ or Ⅱ fungi, suggesting that there may be two biotypes in B. cactivora with different genotypes but for pathogenicity in Korea. Keywords: Alternaria alternata, A. tenuissima, Bipolaris cactivora, Cactus, Colletotrichum gloeosporioides, Glomerella cinglata, RAPD (random amplified polymorphic DNA) | en |
dc.format.extent | xii, 96 leaves | en |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | en |
dc.subject | 선인장 | en |
dc.subject | Alternaria alternata | en |
dc.subject | 탄저병균 | en |
dc.subject | A. tenuissima | en |
dc.subject | 점무늬병균 | en |
dc.subject | Bipolaris cactivora | en |
dc.subject | 줄기썩음병균 | en |
dc.subject | Cactus | en |
dc.subject | Colletotrichum gloeosporioides | en |
dc.subject | Glomerella cinglata | en |
dc.subject | Rapd (random amplified polymorphic dna) | en |
dc.title | 한국에서의 선인장 줄기곰팡이병 : 새로운 병원균과 줄기썩음병균 Bipolaris cactivora의 잠재적 분화형 | en |
dc.type | Thesis | en |
dc.contributor.department | 농생명공학부 | - |
dc.description.degree | Master | en |
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