S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Calcium Hydroxide Inactivates Lipoteichoic Acid from Enterococcus faecalis
- Baik, Jung Eun; Kum, Kee-Yeon; Yun, Cheol-Heui; Lee, Jin-Kyung; Lee, Kangseok; Kim, Kack Kyun; Han, Seung Hyun
- Issue Date
- J Endod. 2008;34(11):1355-1359
- Apical periodontitis; calcium hydroxide; endodontic medicament; Enterococcus faecalis; lipoteichoic acid; Toll-like receptor 2
- Calcium hydroxide is a widely used endodontic medicament for eliminating viable bacteria and inactivating virulence factors. Enterococcus faecalis, a pathogenic gram-positive bacterium, has been associated with refractory apical periodontitis. Because lipoteichoic acid (LTA) is a major virulence factor of gram-positive bacteria, we examined whether calcium hydroxide could detoxify LTA from E. faecalis. An enzyme-linked immunosorbent assay showed that calcium hydroxide–killed E. faecalis was less potent than heat-killed bacteria in stimulating the release of tumor necrosis factor–α by a murine macrophage line, RAW 264.7 (P < 0.05). Pretreatment of LTA with calcium hydroxide remarkably abrogated the ability of LTA to induce the release of tumor necrosis factor–α (P < 0.05). Furthermore, calcium hydroxide–treated LTA was not able to stimulate Toll-like receptor 2, which recognizes functionally intact LTA. These results suggest that calcium hydroxide could detoxify LTA, resulting in attenuation of the inflammatory responses to E. faecalis and its LTA.
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