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Glutamate-induced Cobalt Uptake Elicited by Kainate Receptors in RatTaste Bud Cells

Cited 13 time in Web of Science Cited 12 time in Scopus
Authors
Chung, Ki-Myung; Lee, Seung-Pyo; Lee, Sang-Bok; Heur, Ryang; Cho, Young-Kyung; Lee, Cil-Han; Jung, Hye-Young; Chung, Se-Hwan; Kim, Kyung-Nyun
Issue Date
2005
Publisher
Oxford University Press
Citation
CHEMICAL SENSES 30, 137-143
Keywords
AMPAcobalt stainingfoliate papillaglutamate receptorskainatetaste bud
Abstract
Glutamate-induced cobalt uptake reveals non-N-methyl-D-aspartate (non-NMDA) glutamate receptors (GluRs) in rat taste bud cells. However, it is not known which type of non-NMDA glutamate receptors is involved. We used a cobalt staining technique combined with pharmacological tests for kainate or -amino-3-hydroxy-5-methyl-isoxazole-propionic acid (AMPA) receptors and/or immunohistochemistry against subunits of GluRs to examine the presence of non-NMDA receptors in rat foliate tastebud cells. Cobalt uptake into taste cells was elicited by treating taste buds with glutamate, kainate or SYM 2081, a kainate receptor agonist. Treating taste buds with AMPA or fluorowillardiine did not stimulate significant cobalt uptake. Moreover, 6-cyano-7-nitro-quinoxaline-2, 3-dione significantly reduced cobalt staining elicited by glutamate or kainate receptor agonists, but SYM 2206, an AMPA receptor antagonist, did not. Immunohistochemistry against subunits of GluRs reveals GluR6 and KA1-like immunoreactivity. Moreover, most glutamate-induced cobalt-stained cells showed GluR6 and KA1-like immunoreactivity. These results suggest that glutamate-induced cobalt uptake in taste cells occurs mainly via kainate type GluRs.
ISSN
0379-864X
Language
English
URI
https://hdl.handle.net/10371/68601
DOI
https://doi.org/10.1093/chemse/bji009
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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