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Bupivacaine induces apoptosis via ROS in the schwann cell line

Cited 93 time in Web of Science Cited 95 time in Scopus
Authors
Park, C.J.; Park, S.A.; Yoon, T.G.; Lee, S.J.; Yum, K.W.; Kim, H.J.
Issue Date
2005
Publisher
American and International Associations for Dental
Citation
J Dent Res 84(9):852-857
Keywords
apoptosisbupivacaineneurotoxicityreactive oxygen speciesSchwann cell
Abstract
Local anesthetics have been generally accepted as being safe. However, recent clinical trials and basic studies have provided strong evidence for the neurotoxicity of local anesthetics, especially through apoptosis. We hypothesized that local anesthetics cause neural complications through Schwann cell apoptosis. Among local anesthetics tested on the Schwann cell line, RT4-D6P2T, bupivacaine significantly induced cell death, measured by the methyl tetrazolium (MTT) assay, in a dose- (LD50 = 476 µM) and time-dependent manner. The bupivacaine-induced generation of reactive oxygen species (ROS), which was initiated within 5 hrs and preceded the activation of caspase-3 and poly ADP-ribose polymerase (PARP) degradation, was suggested to trigger apoptosis, exhibited by Hoechst 33258 nuclear staining and DNA fragmentation. Furthermore, concomitant block of ROS by anti-oxidants significantly inhibited bupivacaine-induced apoptosis. Among the local anesthetics for peripheral neural blocks, bupivacaine induced apoptosis in the Schwann cell line, which may be associated with ROS production.
ISSN
0022-0345
Language
English
URI
http://hdl.handle.net/10371/68917
DOI
https://doi.org/10.1177/154405910508400914
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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