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Effect of magnetic field on the fibronectin adsorption, cell attachment and proliferation on titanium surface

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dc.contributor.authorKim, Hyo Jin-
dc.contributor.authorChang, Ik Tae-
dc.contributor.authorHeo, Seong Joo-
dc.contributor.authorKoak, Jai Young-
dc.contributor.authorKim, Seong Kyun-
dc.contributor.authorJang, Jun Hyeog-
dc.date.accessioned2010-08-04T23:00:43Z-
dc.date.available2010-08-04T23:00:43Z-
dc.date.issued2005-10-
dc.identifier.citationClin Oral Implants Res. 16(5):557-562en
dc.identifier.issn0905-7161-
dc.identifier.urihttp://hdl.handle.net/10371/68968-
dc.description.abstractObjectives: We studied the effect of various static magnetic fields (SMFs) on the adsorption of specific recombinant fibronectin (FN) peptide (hFNIII9-10) on the titanium surface. Furthermore, the responses of human osteosarcoma TE-85 cells in the SMF were observed.

Material and methods: Various magnetic fields – 1, 2, 3, 5, 7, 10 mT – were established by controlling the distance from Nd–Fe–B magnet to the disks. For FN adsorption experiment, machined titanium disks were incubated in 1 μM hFNIII9-10 at 37°C overnight under magnetic field. The adsorbed hFNIII9-10 was measured as optical density (OD). For attachment study, TE-85 cells were incubated for 2 h on the hFNIII9-10 coated machined titanium disks and OD values were measured. As for proliferation study, titanium disks were incubated for 48 h after washing unattached cells in 2 h. The amount of proliferated TE-85 cell was also measured as OD value. Attachments of TE-85 cells under various intensities of magnetic field were observed using a scanning electron microscope.

Results: The amount of adsorbed hFNIII9-10 showed no significant difference between control (0 mT) and six experimental groups (1, 2, 3, 5, 7, 10 mT). However, TE-85 cells attached significantly higher in groups of 1, 2, 5, 10 mT than in control group (P=0). Cell attachment in groups of 3, 7 mT showed no significant difference with that of control group. TE-85 cells were observed to attach through filopodia. Especially in 1 mT, flattened cells were predominant. In proliferation assay, 1 mT stimulated TE-85 cells showed significantly higher proliferation than those in 2, 3 and 7 mT (P=0).

Conclusion: Magnetic fields under 10 mT did not influence FN adsorption on the titanium surface. However, a significant effect was found on cell attachment and proliferation.
en
dc.description.sponsorshipThis work was
supported by a grant from the Korean
Health 21 R&D project, Ministry of
Health & Welfare, Korea (02-PJ3-PG6-
EV11-0002).
en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.subjectcell attachmenten
dc.subjectcell proliferationen
dc.subjectfibronectinen
dc.subjectmagnetic fielden
dc.subjecttitaniumen
dc.titleEffect of magnetic field on the fibronectin adsorption, cell attachment and proliferation on titanium surfaceen
dc.typeArticleen
dc.contributor.AlternativeAuthor김효진-
dc.contributor.AlternativeAuthor장익태-
dc.contributor.AlternativeAuthor허성주-
dc.contributor.AlternativeAuthor곽재영-
dc.contributor.AlternativeAuthor김성균-
dc.contributor.AlternativeAuthor장준형-
dc.identifier.doi10.1111/j.1600-0501.2005.01164.x-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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