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Effect of magnetic field on the fibronectin adsorption, cell attachment and proliferation on titanium surface
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hyo Jin | - |
dc.contributor.author | Chang, Ik Tae | - |
dc.contributor.author | Heo, Seong Joo | - |
dc.contributor.author | Koak, Jai Young | - |
dc.contributor.author | Kim, Seong Kyun | - |
dc.contributor.author | Jang, Jun Hyeog | - |
dc.date.accessioned | 2010-08-04T23:00:43Z | - |
dc.date.available | 2010-08-04T23:00:43Z | - |
dc.date.issued | 2005-10 | - |
dc.identifier.citation | Clin Oral Implants Res. 16(5):557-562 | en |
dc.identifier.issn | 0905-7161 | - |
dc.identifier.uri | https://hdl.handle.net/10371/68968 | - |
dc.description.abstract | Objectives: We studied the effect of various static magnetic fields (SMFs) on the adsorption of specific recombinant fibronectin (FN) peptide (hFNIII9-10) on the titanium surface. Furthermore, the responses of human osteosarcoma TE-85 cells in the SMF were observed.
Material and methods: Various magnetic fields – 1, 2, 3, 5, 7, 10 mT – were established by controlling the distance from Nd–Fe–B magnet to the disks. For FN adsorption experiment, machined titanium disks were incubated in 1 μM hFNIII9-10 at 37°C overnight under magnetic field. The adsorbed hFNIII9-10 was measured as optical density (OD). For attachment study, TE-85 cells were incubated for 2 h on the hFNIII9-10 coated machined titanium disks and OD values were measured. As for proliferation study, titanium disks were incubated for 48 h after washing unattached cells in 2 h. The amount of proliferated TE-85 cell was also measured as OD value. Attachments of TE-85 cells under various intensities of magnetic field were observed using a scanning electron microscope. Results: The amount of adsorbed hFNIII9-10 showed no significant difference between control (0 mT) and six experimental groups (1, 2, 3, 5, 7, 10 mT). However, TE-85 cells attached significantly higher in groups of 1, 2, 5, 10 mT than in control group (P=0). Cell attachment in groups of 3, 7 mT showed no significant difference with that of control group. TE-85 cells were observed to attach through filopodia. Especially in 1 mT, flattened cells were predominant. In proliferation assay, 1 mT stimulated TE-85 cells showed significantly higher proliferation than those in 2, 3 and 7 mT (P=0). Conclusion: Magnetic fields under 10 mT did not influence FN adsorption on the titanium surface. However, a significant effect was found on cell attachment and proliferation. | en |
dc.description.sponsorship | This work was
supported by a grant from the Korean Health 21 R&D project, Ministry of Health & Welfare, Korea (02-PJ3-PG6- EV11-0002). | en |
dc.language.iso | en | en |
dc.publisher | Wiley-Blackwell | en |
dc.subject | cell attachment | en |
dc.subject | cell proliferation | en |
dc.subject | fibronectin | en |
dc.subject | magnetic field | en |
dc.subject | titanium | en |
dc.title | Effect of magnetic field on the fibronectin adsorption, cell attachment and proliferation on titanium surface | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김효진 | - |
dc.contributor.AlternativeAuthor | 장익태 | - |
dc.contributor.AlternativeAuthor | 허성주 | - |
dc.contributor.AlternativeAuthor | 곽재영 | - |
dc.contributor.AlternativeAuthor | 김성균 | - |
dc.contributor.AlternativeAuthor | 장준형 | - |
dc.identifier.doi | 10.1111/j.1600-0501.2005.01164.x | - |
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