S-Space College of Veterinary Medicine (수의과대학) Dept. of Veterinary Medicine (수의학과) Journal Papers (저널논문_수의학과)
Regulation of transduction efficiency by pegylation of baculovirus vector in vitro and in vivo
- Issue Date
- Journal of Biotechnology, Vol.125 No.1, pp.104-109
- In this study, poly(ethylene glycol) (PEG) was coupled to baculovirus to regulate transduction efficiency of baculovirus in vitro and in vivo. The degree of pegylation in virions was measured by the loss of free amines via a fluorescamine-based assay. The efficiency of green fluorescent protein (GFP) expression was used to monitor transduction efficiency. As the results, the transduction efficiency in pegylated baculovirus was decreased with an increase of pegylation in baculovirus in vitro and in vivo. Interestingly, the transduction efficiency of the pegylated baculovirus for the lung and brain was increased compared with baculovirus itself possibly owing to increased stability of baculovirus by pegylation. (c) 2006 Elsevier B.V. All rights reserved.
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