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Regulation of transduction efficiency by pegylation of baculovirus vector in vitro and in vivo

Cited 27 time in Web of Science Cited 28 time in Scopus
Issue Date
2006-08
Publisher
Elsevier
Citation
Journal of Biotechnology, Vol.125 No.1, pp.104-109
Keywords
pegylationbaculovirusgreen fluorescent protein (GFP)transduction efficiency
Abstract
In this study, poly(ethylene glycol) (PEG) was coupled to baculovirus to regulate transduction efficiency of baculovirus in vitro and in vivo. The degree of pegylation in virions was measured by the loss of free amines via a fluorescamine-based assay. The efficiency of green fluorescent protein (GFP) expression was used to monitor transduction efficiency. As the results, the transduction efficiency in pegylated baculovirus was decreased with an increase of pegylation in baculovirus in vitro and in vivo. Interestingly, the transduction efficiency of the pegylated baculovirus for the lung and brain was increased compared with baculovirus itself possibly owing to increased stability of baculovirus by pegylation. (c) 2006 Elsevier B.V. All rights reserved.
ISSN
0168-1656
Language
English
URI
https://hdl.handle.net/10371/6898
DOI
https://doi.org/10.1016/j.jbiotec.2006.02.015
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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