Browse

The effecet of UNCL inactivation on the expression of mechanical stress related genes in cultured human PDL fibroblasts

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors
Park, Joo-Cheol; Choi, Yong-Seok; Jang, Hyun-Sun; Lee, Dong-Seol; Kim, Heung-Joong; Park, Jong-Tae; Bae, Hyun-Sook
Issue Date
2008-06-31
Publisher
Korean Acadamy of Oral Biology
Citation
International Journal of Oral Biology, 33(2): 51-58
Keywords
UNCLMechanical stressPDL fibroblastInactivation
Abstract
A mutation of UNCL, an inner nuclear membrane RNAbinding
protein, has been found to eliminate mechanotransduction
in Drosophila. UNCL is expressed in human
periodontal tissue including in periodontal ligament (PDL)
fibroblasts. However, it is unclear how a mechanical
stimulus is translated into cellular responses in PDL
fibroblasts. The aim of this study was to evaluate the effect
of UNCl on mechanical stress related genes in PDL
fibroblasts in response to mechanical stress. The mRNA of
TGF-β, COX-2, and MMP-2 was up-regulated after UNCL
inactivation in PDL fibroblasts under the compression
force. Under the tensile force, inactivation of UNCL
decreased the expression of Biglycan, RANKL, MMP-2,
and TIMP-2 mRNAs while it increased the expression of
TIMP-1. p38-MAPK was expressed in PDL fibroblasts
under compression forces whereas phospho-ERK1/2, p65-
NFkB, and c-fos were expressed under tension forces. The
expression and phosphorylation of the mechanical stress
related genes, kinases, and transcription factors were
changed according to the types of stress. Furthermore, most
of them were regulated by the inactivation of UNCL. This
suggests that UNCL is involved in the regulation of
mechanical stress related genes through the signaling
pathway in PDL fibroblasts.
ISSN
1226-7155
Language
English
URI
https://hdl.handle.net/10371/69619
Files in This Item:
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse