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Tetraspanin CD9 regulates osteoclastogenesis via regulation of p4442 MAPK activity

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Authors
Kim, Gwan-Shik; Yi, TacGhee; Kim, Hye-Jin; Cho, Je-Yoel; Woo, Kyung Mi; Ryoo, Hyun-Mo; Baek, Jeong-Hwa
Issue Date
2006-08
Publisher
Elsevier
Citation
Biochemical and Biophysical Research Communications 347 (2006) 178-184
Keywords
OsteoclastogenesisMultinuclear osteoclastCD9KMC8p44/42 MAPK
Abstract
Tetraspanin CD9 has been shown to regulate cell–cell fusion in sperm-egg fusion and myotube formation. However, the role of CD9 in osteoclast, another multinucleated cell type, is not still clear. Therefore, we investigated the role of CD9 in osteoclast differentiation. CD9 was expressed in osteoclast lineage cells and its expression level increased during the progression of RANKL-induced osteoclastogenesis. KMC8, a neutralizing antibody specific to CD9, significantly suppressed RANKL-induced multinucleated osteoclast formation and the mRNA expression of osteoclast differentiation marker genes. To define CD9-regulated osteoclastogenic signaling pathway, MAPK pathways were examined. KMC8 induced long-term phosphorylation of p44/42 MAPK, but not of p38 MAPK. Constitutive activation of p44/42 MAPK by overexpressing constitutive-active mutant of MEK1 almost completely blocked osteoclast differentiation. Taken together, these results suggest that CD9 expressed on osteoclast lineage cells might positively regulate osteoclastogenesis via the regulation of p44/42 MAPK activity.
ISSN
0006-291X
Language
English
URI
http://hdl.handle.net/10371/69737
DOI
https://doi.org/10.1016/j.bbrc.2006.06.061
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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