S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
"Immunoregulatory effects of extracts from the stem bark of Albizzia julibrissin Durazz
- Choi, Youngnim; Kim, Jeong J; Lee, Byung E; Jung, Dong S; Kim, Se Won; Ko, Seon Yle; Baek, Dong Heon; Chang, Sun Hwa
- Issue Date
- Korean Acadamy of Oral Biology
- International Journal of Oral Biology 29:137-144
- disease modifying anti-rheumatic(DMARD); albizzia julibrissin; activated T cell; apoptosis; caspase-8
- Although there are many disease modifying antirheumatic drugs (DMARDs) available, more safe and effective reagents have yet to be developed. Activated T cells make good targets for DMARD therapy. Therefore, we screened over fifty herbal extracts, and identified that water extract from the stem bark of Albizzia julibrissin selectively killed activated T cells but not naive splenocytes, in vitro. Wb3-3, obtained by further extraction and column chromatography to maximize selective activity, was used for further investigation. Annexin-V and caspase activity assay revealed that WB3-3 killed activated T cells by inducing apoptosis, which was mediated through mainly caspase-8 rather than caspase-9. The effect of WB3-3 in vivo was conlirmed by the elimination of V8- but no V6-T cells following activation with Streptococcal Enterotoxin B (SEB). Furthermore, WB3-3 inhibited neither CD25 upregulationnor proliferative response, and rather increased IL-2 secretion during Con A activation of naive T cells.Our results imply the potential of WB3-3 to include a lead compound for a new immunoregulatory drug.