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Identification of antigenic peptide recognized by the anti-JL1 leukemia-specific monoclonal antibody from combinatorial peptide phage display libraries

Cited 11 time in Web of Science Cited 12 time in Scopus
Authors
Chung, Junho; Park, Sojung; Kim, Dongjo; Rhim, Junghyo; Kim, Ikjung; Choi, Inhak; Yi, Kyesook; Ryu, Sungho; Suh, Pannghill; Chung, Doohyun; Bae, Youngmee; Shin, Youngkee; Park, Sunghoe
Issue Date
2002-12
Publisher
Springer
Citation
J Cancer Res Clin Oncol; Vol.128, No.12; pp.641–649
Keywords
Phage displayCombinatorial peptide libraryMimotope-Monoclonal antibodyJL1 antigen
Abstract
PURPOSE: In the present study an antigen-mimetic peptide of the anti-JL1 leukemia-specific monoclonal antibody (mAb) was identified and characterized.
METHODS: From combinatorial peptide phage display libraries displaying the random linear heptapeptides and dodecapeptides, we selected clones with affinity to anti-JL1 mAb through repeated rounds of panning on a mAb-coated ELISA plate. The antigenicity and immunogenicity of the peptide epitopes were then studied using chemically synthesized peptides.
RESULTS: The selected clones had the LXPSIP consensus sequence. Two synthetic peptides LPPSIPFGLTVGGGGS and LLPSIPNQAYLGGGGS specifically reacted with anti-JL1 mAb in ELISA. These two peptides were found to inhibit the interaction between anti-JL1 mAb and JL1 antigen-positive Molt-4 cells. Although the immune sera raised against the keyhole limpet hemocyanin-conjugated peptides failed to react with Molt-4 cells, it showed strong reactivity to the peptide epitope. However, one mAb raised by peptide immunization successfully bound to Molt-4 cells. CONCLUSION: An epitope-mimetic peptide of anti-JL1 mAb was found using combinatorial peptide phage display libraries. It induced strong humoral response against itself, but only a limited fraction of this humoral response was cross-reactive with the original JL1 antigen.
ISSN
0171-5216
Language
English
URI
http://hdl.handle.net/10371/73499
DOI
https://doi.org/10.1007/s00432-002-0390-x
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College of Pharmacy (약학대학)Dept. of Pharmacy (약학과)Journal Papers (저널논문_약학과)
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