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Nanowire-Based Delivery of Escherichia coli O157 Shiga Toxin 1 A Subunit into Human and Bovine Cells

Cited 29 time in Web of Science Cited 32 time in Scopus
Authors
Kwon, Nam Hoon; Beaux II, Miles F.; Ebert, Chad; Wang, Lidong; Lassiter, Brian E.; Park, Yong Ho; McIlroy, David N.; Hovde, Carolyn J.; Bohach, Gregory A.
Issue Date
2007-07-27
Publisher
American Chemical Society
Citation
Nano Lett. 2007, 7, 2718-2723
Abstract
Silica nanowires (NWs) were used to introduce the Shiga toxin type 1 A subunit (StxA1) into cultured bovine and human epithelial cells. We
extended technology developed in our laboratories that employs fibronectin (Fn) to induce integrin-mediated uptake of NWs by coating NWs
with StxA1 and Fn. The bonding strengths of Fn and StxA1 to the surface of NWs were measured by X-ray photoelectron spectroscopy. This
technique demonstrated complex interactions between Fn, StxA1, and the NWs. Neutral red cytotoxicity assays and field emission scanning
electron microscopy confirmed that the NW-StxA1-Fn complexes were effectively internalized and caused cell death. This indicates that NWs
can carry StxA1 and potentially other toxic or therapeutic agents into eukaryotic cells. Ongoing studies include improved functionalizing of
NWs aimed at increasing internalization efficiency and substituting ligands for specific cell targeting.
ISSN
1530-6984
Language
English
URI
https://hdl.handle.net/10371/7392
DOI
https://doi.org/10.1021/nl071179f
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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