S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Microglial toll-like receptor 2 contributes to kainic acid-induced glial activation and hippocampal neuronal cell death
- Hong, Jinpyo; Cho, Ik-Hyun; Kwak, Kyung Il; Suh, Eun Cheng; Seo, Jinsoo; Min, Hyun Jung; Choi, Se-Young; Kim, Chong-Hyun; Park, Seung Hwa; Jo, Eun-Kyeong; Lee, Soojin; Lee, Kyung Eun; Lee, Sung Joong
- Issue Date
- Journal of Biological Chemistry 285,39447-39457
- Recent studies indicate that Toll-like receptors (TLRs), originally identified as infectious agent receptors, also mediate sterile inflammatory responses during tissue damage. In this study, we investigated the role of TLR2 in excitotoxic hippocampal cell death using TLR2 knock-out (KO) mice. TLR2 expression was up-regulated in microglia in the ipsilateral hippocampus of kainic acid (KA)-injected mice. KA-mediated hippocampal cell death was significantly reduced in TLR2 KO mice compared with wild-type (WT) mice. Similarly, KA-induced glial activation and proinflammatory gene expression in the hippocampus were compromised in TLR2 KO mice. In addition, neurons in organotypic hippocampal slice cultures (OHSCs) from TLR2 KO mouse brains were less susceptible to KA excitotoxicity than WT OHSCs. This protection is partly attributed to decreased expression of proinflammatory genes, such as TNF-α and IL-1β in TLR2 KO mice OHSCs. These data demonstrate conclusively that TLR2 signaling in microglia contributes to KA-mediated innate immune responses and hippocampal excitotoxicity.
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