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Inhibition of L-Type amino acid transporter modulates the expression of cell cycle regulatory factors in KB oral cancer cells

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Authors
Kim, Chun Sung; Moon, In-Sung; Park, Ju-Hyun; Shin, Woo-Cheol; Chun, Hong Sung; Lee, Sook-Young; Kook, Joong-Ki; Kim, Heung-Joong; Park, Joo-Cheol; Endou, Hitoshi; Kanai, Yoshikatsu; Lee, Byung-Kwon; Kim, Do Kyung
Issue Date
2010-07
Publisher
Pharmaceutical Society of Japan
Citation
Biological and Pharmaceutical Bulletin 33,1117-1121 (2010)
Keywords
L-type amino acid transportercancer cellcell cycle regulatory factor2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid
Abstract
The purpose of this study was to examine the effect of 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH), an inhibitor of L-type amino acid transporters, on the cell growth suppression in KB human oral cancer cells and to study the roles of cell cycle regulatory factors in the BCH-induced growth inhibition. The effect of BCH on cell growth suppression and the influence of BCH to cell cycle regulatory factors in KB cell growth inhibition were examined using cell cycle analysis, immunoblotting and immunoprecipitation. The BCH treatment induced cell cycle arrest at G1 phase in KB cells. The expression of cyclin D3 was remarkably decreased by BCH treatment. The BCH inhibited the expression of cyclin-dependent protein kinase 6 (CDK6) in a time-dependent manner. In addition, the expression of CDK inhibitor p27 was increased by BCH treatment in KB cells, but not CDK inhibitors p21 and p15. These results suggest that, in KB cells, the inhibition of LAT1 by BCH causes cell cycle arrest at G1 phase by inhibiting cyclin D3-CDK6 complex whereas increasing expression of a CDK inhibitor p27.
ISSN
0918-6158
Language
English
URI
http://hdl.handle.net/10371/74120
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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