Browse

Swelling-activated Cl- Channels in Human Salivary Gland Acinar Cells

Cited 0 time in Web of Science Cited 0 time in Scopus
Issue Date
2009-09
Publisher
Korean Acadamy of Oral Biology
Citation
Int. J. Oral Biol. 2009;34(3):151-155
Keywords
Cl- channelsRegulatory volume decrease(RVD)HypotonicNPPBDIDS
Abstract
The role of Cl
-
channels in regulatory volume decrease
(RVD) in human salivary gland acinar cells was examined
using a whole-cell patch clamp technique. Human tissues
were obtained from healthy volunteers or from patients
with oromaxillofacial tumors. During the measurements,
K+-free solutions were employed to eliminate contamination
of whole-cell conductance by K+
currents. When the cells
were exposed to a 70% hypotonic solution, outward-rectifying
currents, which were not observed in the resting state, were
found to have significantly increased both in human labial
and parotid gland acinar cells. The amplitudes of the
currents were reduced in a low Cl
-
bath solution. Furthermore,
the addition of 100 μM 5-Nitro-2- (3-phenyl propylamino)
benzoic acid (NPPB) or 100 μM 4,4'-diisothio
cyanatostilbene -2,2'-disulphonic acid (DIDS), known to
partially block Cl
-
channels, significantly inhibited these
currents. Its outward-rectifying current profile, shift in
reversal potential in a low Cl
-
bath solution and pharmacological
properties suggest that this is a Ca
2+-independent,
volume activated Cl
-
current. We conclude therefore that
volume activated Cl
-
channels play a putative role in RVD in
human salivary gland acinar cells.
ISSN
1226-7155
Language
Korean
URI
https://hdl.handle.net/10371/74363
Files in This Item:
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse