Publications
Detailed Information
Swelling-activated Cl- Channels in Human Salivary Gland Acinar Cells
Cited 0 time in
Web of Science
Cited 0 time in Scopus
- Authors
- Issue Date
- 2009-09
- Publisher
- Korean Acadamy of Oral Biology
- Citation
- Int. J. Oral Biol. 2009;34(3):151-155
- Keywords
- Cl- channels ; Regulatory volume decrease(RVD) ; Hypotonic ; NPPB ; DIDS
- Abstract
- The role of Cl
-
channels in regulatory volume decrease
(RVD) in human salivary gland acinar cells was examined
using a whole-cell patch clamp technique. Human tissues
were obtained from healthy volunteers or from patients
with oromaxillofacial tumors. During the measurements,
K+-free solutions were employed to eliminate contamination
of whole-cell conductance by K+
currents. When the cells
were exposed to a 70% hypotonic solution, outward-rectifying
currents, which were not observed in the resting state, were
found to have significantly increased both in human labial
and parotid gland acinar cells. The amplitudes of the
currents were reduced in a low Cl
-
bath solution. Furthermore,
the addition of 100 μM 5-Nitro-2- (3-phenyl propylamino)
benzoic acid (NPPB) or 100 μM 4,4'-diisothio
cyanatostilbene -2,2'-disulphonic acid (DIDS), known to
partially block Cl
-
channels, significantly inhibited these
currents. Its outward-rectifying current profile, shift in
reversal potential in a low Cl
-
bath solution and pharmacological
properties suggest that this is a Ca
2+-independent,
volume activated Cl
-
current. We conclude therefore that
volume activated Cl
-
channels play a putative role in RVD in
human salivary gland acinar cells.
- ISSN
- 1226-7155
- Language
- Korean
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.