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Novel Effect of Biphasic Electric Current on In Vitro Osteogenesis and Cytokine Production in Human Mesenchymal Stromal Cells

Cited 69 time in Web of Science Cited 71 time in Scopus
Authors
Kim, In Sook; Song, Jong Keun; Song, Yun Mi; Cho, Tae Hyung; Lee, Tae Hyung; Lim, Shin Saeng; Kim, Sung June; Hwang, Soon Jung
Issue Date
2009-03-11
Publisher
Mary Ann Liebert
Citation
Tissue Engineering Part A. -Not available-, ahead of print. doi:10.1089/ten.tea.2008.0554
Abstract
Electrical stimulation (ES) can activate diverse biostimulatory responses in a range of tissues. Of various forms of
ES, the application of biphasic electric current (BEC) is a new approach to bone formation. This study is to
investigate the effects and mechanism of action of BEC in osteoblast differentiation and cytokine production in
human mesenchymal stromal cells (hMSCs). Using an in vitro culture system with a modified version of the BEC
stimulator chip used in our previous study, we exposed hMSCs to a 100 Hz ES with a magnitude of
1.5=15 mA=cm2 for 250=25 ms. hMSCs showed increased proliferation during static BEC stimulation for 5 days.
However, alkaline phosphatase activity and calcium deposition were enhanced in hMSCs 7 days after the
stimulation, rather than during the period of ES. BEC induced vascular endothelial growth factor (VEGF) and
BMP-2 production; the former can enhance the proliferation of human umbilical vein endothelial cells in culture
using conditioned media from BEC cultures. Treatment with selective inhibitors of p38 MAPK (SB203580) or Erk
(PD98059), as well as calcium channel blockers (verapamil and nifedipine), reduced the BEC-mediated increase
of VEGF expression and cell proliferation. These findings reveal that BEC is involved in the osteoblast differentiation
of hMSCs through enhancement of cell proliferation and modulation of the local endocrine environment
through VEGF and BMP-2 induction through the activation of MAPK (Erk and p38) and the calcium
channel. Thus, local stimulation using BEC might be most beneficial in promoting osteogenic differentiation of
hMSCs, resulting in enhanced bone formation for bone tissue engineering
ISSN
1937-335X
Language
English
URI
http://hdl.handle.net/10371/7450
DOI
https://doi.org/10.1089/ten.tea.2008.0554
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Electrical and Computer Engineering (전기·정보공학부)Journal Papers (저널논문_전기·정보공학부)
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