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Novel Effect of Biphasic Electric Current on In Vitro Osteogenesis and Cytokine Production in Human Mesenchymal Stromal Cells
Cited 82 time in
Web of Science
Cited 83 time in Scopus
- Authors
- Issue Date
- 2009-03-11
- Publisher
- Mary Ann Liebert
- Citation
- Tissue Engineering Part A. -Not available-, ahead of print. doi:10.1089/ten.tea.2008.0554
- Abstract
- Electrical stimulation (ES) can activate diverse biostimulatory responses in a range of tissues. Of various forms of
ES, the application of biphasic electric current (BEC) is a new approach to bone formation. This study is to
investigate the effects and mechanism of action of BEC in osteoblast differentiation and cytokine production in
human mesenchymal stromal cells (hMSCs). Using an in vitro culture system with a modified version of the BEC
stimulator chip used in our previous study, we exposed hMSCs to a 100 Hz ES with a magnitude of
1.5=15 mA=cm2 for 250=25 ms. hMSCs showed increased proliferation during static BEC stimulation for 5 days.
However, alkaline phosphatase activity and calcium deposition were enhanced in hMSCs 7 days after the
stimulation, rather than during the period of ES. BEC induced vascular endothelial growth factor (VEGF) and
BMP-2 production; the former can enhance the proliferation of human umbilical vein endothelial cells in culture
using conditioned media from BEC cultures. Treatment with selective inhibitors of p38 MAPK (SB203580) or Erk
(PD98059), as well as calcium channel blockers (verapamil and nifedipine), reduced the BEC-mediated increase
of VEGF expression and cell proliferation. These findings reveal that BEC is involved in the osteoblast differentiation
of hMSCs through enhancement of cell proliferation and modulation of the local endocrine environment
through VEGF and BMP-2 induction through the activation of MAPK (Erk and p38) and the calcium
channel. Thus, local stimulation using BEC might be most beneficial in promoting osteogenic differentiation of
hMSCs, resulting in enhanced bone formation for bone tissue engineering
- ISSN
- 1937-335X
- Language
- English
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