S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Physiology (생리학교실) Journal Papers (저널논문_생리학교실)
Identification of TRPM7 channels in human intestinal interstitial cells of Cajal
- Kim, Byung Joo; Park, Kyu Joo; Kim, Hyung Woo; Choi, Seok; So, Insuk; Kim, Seon Jeong; Jeon, Ju-Hong; Chang, In Youb; Jun, Jae Yeoul
- Issue Date
- W J G PRESS
- WORLD JOURNAL OF GASTROENTEROLOGY; Vol.15 46; 5799-5804
- Electrophysiology; Gastrointestinal tract; Human; Interstitial cells of Cajal; TRPM cation channels; Transient receptor potential melastatin-type 7 protein
- AIM: To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 (TRPM7) in the human gastrointestinal (GI) tract. METHODS: Conventional microelectrode techniques were used to record intracellular electrical responses from human GI smooth muscle tissue. Immunohistochemistry was used to identify TRPM7 channels in interstitial cells of Cajal (ICCs). RESULTS: The human GI tract generated slow electrical waves and had ICCs which functioned as pacemaker cells. Flufenamic acid, a nonselective cation channel blocker, and 2-APB (2-aminoethoxydiphenyl borate) and La(3+), TRPM7 channel blockers, inhibited the slow waves. Also, TRPM7 channels were expressed in ICCs in human tissue. CONCLUSION: These results suggest that the human GI tract generates slow waves and that TRPM7 channels expressed in the ICCs may be involved in the generation of the slow waves. (C) 2009 The WJG Press and Baishideng. All rights reserved.