S-Space College of Medicine/School of Medicine (의과대학/대학원) Pathology (병리학전공) Journal Papers (저널논문_병리학전공)
Tissue Expression of Manganese Superoxide Dismutase Is a Candidate Prognostic Marker for Glioblastoma
- Park, Chul-Kee; Jung, Ji Hye; Moon, Min Jeong; Kim, Young-Yim; Park, Sung-Hye; Paek, Sun Ha; Jung, Hee-Won; Cho, Byung-Kyu; Kim, Dong Gyu; Kim, Chae-Yong; Kim, Jin Hyun
- Issue Date
- ONCOLOGY; Vol.77 03월 04일; 178-181
- Proteomics; Long-term survival; Manganese superoxide dismutase; Prognostic factor; Glioblastoma
- Background: Characterization of a rare subgroup of glioblastoma patients who survive for more than 3 years (long-term survival glioblastoma, LTSGBL, patients) may be helpful to identify prognostic factors. Materials and Methods: A molecular-profiling proteomic approach using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to identify prognostic factors associated with glioblastoma by comparing frozen tumor tissue from LTSGBL patients with matched samples from short-term survival glioblastoma (STSGBL) patients. Western blot (WB) analysis, reverse-transcriptase polymerase chain reaction (RT-PCR) and immmunohistochemical (IHC) staining were used for confirmation. Results: Among most candidate spots identified by 2-DE, lack of overexpression of manganese superoxide dismutase (MnSOD) in LTSGBL samples was consistently observed using WB and RT-PCR. Conclusion: These results suggest that MnSOD expression level in tumor tissue is a candidate marker for the prognosis of glioblastoma patients. Copyright (C) 2009 S. Karger AG, Basel
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