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Identification and characterization of proteins in amniotic fluid that are differentially expressed before and after antenatal corticosteroid administration

Cited 8 time in Web of Science Cited 9 time in Scopus
Authors
Lee, JoonHo; Park, Joong Shin; Norwitz, Errol R.; Kim, Byoung Jae; Jun, Jong Kwan; Syn, Hee Chul; Park, Chan-Wook
Issue Date
2010-04
Publisher
MOSBY-ELSEVIER
Citation
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY; Vol.202 4; -
Keywords
Albumintransthyretinsurface-enhanced laser desorption/ionization time-of-flight mass spectrometryantenatal corticosteroidslumicanprothrombin fragment 2amniotic fluid
Abstract
OBJECTIVE: We sought to examine changes in the intraamniotic proteomic environment after the administration of antenatal corticosteroids to women with impending preterm delivery. STUDY DESIGN: Amniotic fluid samples were collected at the time of clinically indicated amniocentesis before and within 7 days of administration of antenatal corticosteroids for impending preterm delivery (n = 12). Proteins differentially expressed before and after corticosteroids were identified by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. They were isolated, characterized, and quantified by fast protein liquid chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, in-gel tryptic digestion, immunodepletion assays, enzyme-linked immunosorbent assay, and electrospray ionization tandem mass spectrometry. RESULTS: Five protein peaks of interest were identified and characterized, all of which were significantly decreased after antenatal corticosteroid administration. These included 2 isoforms of transthyretin, albumin, prothrombin fragment 2, and lumican. CONCLUSION: Four proteins, identified and characterized in amniotic fluid, were differentially expressed with antenatal corticosteroid administration. These data may provide additional insight into the molecular mechanisms by which antenatal corticosteroids prevent neonatal complications.
ISSN
0002-9378
Language
English
URI
http://hdl.handle.net/10371/76825
DOI
https://doi.org/10.1016/j.ajog.2010.01.056
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College of Medicine/School of Medicine (의과대학/대학원)Obstetrics & Gynecology (산부인과전공)Journal Papers (저널논문_산부인과학전공)
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