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Modification of dendritic cells with interferon-γ-inducible protein-10 gene to enhance vaccine potency
DC Field | Value | Language |
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dc.contributor.author | Kang, Tae Heung | - |
dc.contributor.author | Bae, Hyun Cheol | - |
dc.contributor.author | Kim, Seok-Ho | - |
dc.contributor.author | Seo, Su Hong | - |
dc.contributor.author | Choi, Eun Young | - |
dc.contributor.author | Kim, Tae Woo | - |
dc.contributor.author | Seong, Seung-Yong | - |
dc.contributor.author | Son, Sang Wook | - |
dc.date.accessioned | 2012-06-11T02:18:22Z | - |
dc.date.available | 2012-06-11T02:18:22Z | - |
dc.date.issued | 2009-10 | - |
dc.identifier.citation | JOURNAL OF GENE MEDICINE; Vol.11 10; 889-898 | ko_KR |
dc.identifier.issn | 1099-498X | - |
dc.identifier.uri | https://hdl.handle.net/10371/76945 | - |
dc.description.abstract | Background Dendritic cell (DC)-based vaccines have become a promising modality in cancer immunotherapy. However, their ability to initiate tumor antigen-specific T cell immunity is limited in various negative-feedback mechanisms. The rapid down-regulation of chemokines, such as the interferon inducible protein of 10 kDa (IP-10), which chemoattracts activated antigen-specific CD8(+) T cells, would represent negative-feedback regulation. Therefore, we attempted to improve DC vaccine potency by introducing the IP-10 gene retrovirally aiming to replenish the chemoattractive activity of DCs. Methods We introduced IP-10 gene into DC2.4 cells, referred to as DC-IP10, using a retroviral system. Nonsecretable mIP-10-expressing DCs (DC-mIP10) were also prepared to evaluate the effects of secretion in IP-10-mediated modulation of DC biology. Additionally, in vitro and in vivo activation of antigen-specific T lymphocytes and in vivo anti-tumor effects induced by DC-IP10 or DC-mIP10 were determined. Results The modification of DC2.4 cells with the IP-10 gene resulted in the secretion of functionally chemoattractive IP-10 and, unexpectedly, a significant up-regulation of surface expression in co-stimulatory molecules, such as CD40 and CD80, compared to that of DCs with vector control (DC-no insert). DC-mIP10 also displayed the partially matured phenotypes but failed to recruit antigen-specific T cells in an in vitro cell culture system. Consistently, DC-IP10 generated more tumor antigen-specific CD8+ T cells and stronger anti-tumor effects in vaccinated mice than did control DCs and DC-mIP10. Conclusions The results obtained provide the groundwork for a future clinical translation of the chemokine-based genetic modification of DCs to increase their vaccine potency. Copyright (C) 2009 John Wiley & Sons, Ltd. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | JOHN WILEY & SONS LTD | ko_KR |
dc.subject | IP-10 | ko_KR |
dc.subject | dendritic cell | ko_KR |
dc.subject | cancer vaccine | ko_KR |
dc.subject | chemokine | ko_KR |
dc.subject | immunotherapy | ko_KR |
dc.title | Modification of dendritic cells with interferon-γ-inducible protein-10 gene to enhance vaccine potency | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 강태흥 | - |
dc.contributor.AlternativeAuthor | 배현철 | - |
dc.contributor.AlternativeAuthor | 김석호 | - |
dc.contributor.AlternativeAuthor | 서수홍 | - |
dc.contributor.AlternativeAuthor | 손상욱 | - |
dc.contributor.AlternativeAuthor | 최은영 | - |
dc.contributor.AlternativeAuthor | 성승용 | - |
dc.contributor.AlternativeAuthor | 김태우 | - |
dc.identifier.doi | 10.1002/jgm.1371 | - |
dc.citation.journaltitle | JOURNAL OF GENE MEDICINE | - |
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