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Enhancement of dendritic cell-based vaccine potency by anti-apoptotic siRNAs targeting key pro-apoptotic proteins in cytotoxic CD8(+) T cell-mediated cell death
DC Field | Value | Language |
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dc.contributor.author | Kim, Jin Hee | - |
dc.contributor.author | Kang, Tae Heung | - |
dc.contributor.author | Noh, Kyung Hee | - |
dc.contributor.author | Bae, Hyun Cheol | - |
dc.contributor.author | Yoo, Young Do | - |
dc.contributor.author | Kim, Tae Woo | - |
dc.contributor.author | Seong, Seung-Yong | - |
dc.contributor.author | Kim, Seok-Ho | - |
dc.date.accessioned | 2012-06-11T02:43:20Z | - |
dc.date.available | 2012-06-11T02:43:20Z | - |
dc.date.issued | 2009-01-29 | - |
dc.identifier.citation | IMMUNOLOGY LETTERS; Vol.122 1; 58-67 | ko_KR |
dc.identifier.issn | 0165-2478 | - |
dc.identifier.uri | https://hdl.handle.net/10371/76953 | - |
dc.description.abstract | Dendritic cells (DCs) have become an important measure for the treatment of malignancies. Current DC preparations, however, generate short-lived DCs because they are subject to cell death from various apoptotic pressures. Antigen-specific CD8(+) cytotoxic T lymphocytes (CTLs) is one of the main obstacles to limit the DC-mediated immune priming since CTLs can recognize the target antigen expressing DCs as target cells and kill the DCs. CTLs secret perforin and serine protease granzymes during CTL killing. Perforin and serine protease granzymes induce the release of a number of mitochondrial pro-apoptotic factors, which are controlled by members of the BCL-2 family, such as BAK, BAX and BIM. FasL linking to Fas on DCs triggers the activation of caspase-8, which eventually leads to mitochondria-mediated apoptosis via truncation of BID. In this study, we tried to enhance the DC priming capacity by prolonging DC survival using anti-apoptotic siRNA targeting these key pro-apoptotic molecules in CTL killing. Human papillomavirus (HPV)-16 E7 antigen presenting DCs that were transfected with these anti-apoptotic siRNAs showed increased resistance to T cell-mediated death, leading to enhanced V-specific CD8(+) T cell activation in vitro and in vivo. Among them, siRNA targeting BIM (siBIM) generated strongest P-specific E7-specific CD8(+)T cell immunity. More importantly, vaccination with E7 presenting DCs transfected with siBIM was capable of generating a marked therapeutic effect in vaccinated mice. Our data indicate that ex vivo manipulation of DCs with siBIM may represent a plausible strategy for enhancing dendritic cell-based vaccine potency. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | ELSEVIER SCIENCE BV | ko_KR |
dc.subject | Dendritic cell | ko_KR |
dc.subject | Immunotherapy | ko_KR |
dc.subject | siRNA | ko_KR |
dc.subject | BIM | ko_KR |
dc.subject | Sig/E7/LAMP-1 | ko_KR |
dc.subject | HPV E7 | ko_KR |
dc.title | Enhancement of dendritic cell-based vaccine potency by anti-apoptotic siRNAs targeting key pro-apoptotic proteins in cytotoxic CD8(+) T cell-mediated cell death | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 김진희 | - |
dc.contributor.AlternativeAuthor | 강태흥 | - |
dc.contributor.AlternativeAuthor | 노경희 | - |
dc.contributor.AlternativeAuthor | 배현철 | - |
dc.contributor.AlternativeAuthor | 김석호 | - |
dc.contributor.AlternativeAuthor | 유영도 | - |
dc.contributor.AlternativeAuthor | 성승용 | - |
dc.contributor.AlternativeAuthor | 김태우 | - |
dc.identifier.doi | 10.1016/j.imlet.2008.12.006 | - |
dc.citation.journaltitle | IMMUNOLOGY LETTERS | - |
dc.description.citedreference | Jorgensen TN, 2007, J IMMUNOL, V179, P3417 | - |
dc.description.citedreference | Chen M, 2007, BLOOD, V109, P4360, DOI 10.1182/blood-2006-11-056424 | - |
dc.description.citedreference | Kang TH, 2007, INT J CANCER, V120, P1696, DOI 10.1002/ijc.22377 | - |
dc.description.citedreference | Kang TH, 2006, IMMUNOL LETT, V106, P126, DOI 10.1016/j.imlet.2006.05.004 | - |
dc.description.citedreference | Yang JP, 2006, P NATL ACAD SCI USA, V103, P147, DOI 10.1073/pnas.0509054103 | - |
dc.description.citedreference | Peng SW, 2005, HUM GENE THER, V16, P584 | - |
dc.description.citedreference | Strasser A, 2005, NAT REV IMMUNOL, V5, P189, DOI 10.1038/nri1568 | - |
dc.description.citedreference | Waterhouse NJ, 2005, J BIOL CHEM, V280, P4476, DOI 10.1074/jbc.M410985200 | - |
dc.description.citedreference | Kim TW, 2005, CANCER RES, V65, P309 | - |
dc.description.citedreference | Hou WS, 2004, NAT IMMUNOL, V5, P583, DOI 10.1038/ni1071 | - |
dc.description.citedreference | Figdor CG, 2004, NAT MED, V10, P475, DOI 10.1038/nm1039 | - |
dc.description.citedreference | Kim TW, 2003, J IMMUNOL, V171, P2970 | - |
dc.description.citedreference | Kim TW, 2003, J CLIN INVEST, V112, P109, DOI 10.1172/JCI200317293 | - |
dc.description.citedreference | Engleman EG, 2003, SEMIN ONCOL, V30, P23, DOI 10.1016/S0093-7754(03)00229-X | - |
dc.description.citedreference | Esposti MD, 2003, BIOCHEM BIOPH RES CO, V304, P455, DOI 10.1016/S0006-291X(03)00617-X | - |
dc.description.citedreference | Opferman JT, 2003, NAT IMMUNOL, V4, P410 | - |
dc.description.citedreference | Schuler G, 2003, CURR OPIN IMMUNOL, V15, P138, DOI 10.1016/S0952-7915(03)00015-3 | - |
dc.description.citedreference | Liu K, 2002, J EXP MED, V196, P1091, DOI 10.1084/jem.20021215 | - |
dc.description.citedreference | Langenkamp A, 2002, EUR J IMMUNOL, V32, P2046 | - |
dc.description.citedreference | Russell JH, 2002, ANNU REV IMMUNOL, V20, P323 | - |
dc.description.citedreference | Medema JP, 2001, J EXP MED, V194, P657 | - |
dc.description.citedreference | Ronchese F, 2001, J EXP MED, V194, pF23 | - |
dc.description.citedreference | Cheng EHYA, 2001, MOL CELL, V8, P705 | - |
dc.description.citedreference | Zong WX, 2001, GENE DEV, V15, P1481 | - |
dc.description.citedreference | Hung CF, 2001, CANCER RES, V61, P3698 | - |
dc.description.citedreference | Derby MA, 2001, J IMMUNOL, V166, P1690 | - |
dc.description.citedreference | Wang TL, 2000, GENE THER, V7, P726 | - |
dc.description.citedreference | Hermans IF, 2000, J IMMUNOL, V164, P3095 | - |
dc.description.citedreference | Jacotot E, 1999, ANN NY ACAD SCI, V887, P18 | - |
dc.description.citedreference | Oehen S, 1998, J IMMUNOL, V161, P5338 | - |
dc.description.citedreference | Lin KY, 1996, CANCER RES, V56, P21 | - |
dc.description.citedreference | INABA K, 1992, J EXP MED, V176, P1693 | - |
dc.description.tc | 16 | - |
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