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Association of Increased Pulmonary Interleukin-6 with the Priming Effect of Intra-Amniotic Lipopolysaccharide on Hyperoxic Lung Injury in a Rat Model of Bronchopulmonary Dysplasia

Cited 16 time in Web of Science Cited 17 time in Scopus
Authors
Kim, Do-Hyun; Choi, Chang Won; Kim, Ee-Kyung; Kim, Han-Suk; Choi, Jung-Hwan; Yang, Eun Gyeong; Lee, Myong Jin; Kim, Beyong Il
Issue Date
2010
Publisher
KARGER
Citation
NEONATOLOGY; Vol.98 1; 23-32
Keywords
AlveolarizationBronchopulmonary dysplasiaInflammationInterleukin-6
Abstract
Background: The authors previously demonstrated the priming effect of intra-amniotic lipopolysaccharide (LPS) on hyperoxic lung injury in a rat model of bronchopulmonary dysplasia (BPD). Objectives: To investigate the mechanism underlying this priming effect by determining biochemical profiles in a rat model of BPD. Methods: The rat model involved intra-amniotic LPS administration and postnatal hyperoxia (85%). The mRNA expressions of interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), VEGF receptor-2 (VEGFR-2), basic fibroblast growth factor (bFGF), and transforming growth factor beta(1) (TGF-beta(1)), as well as the protein levels of IL-6, VEGF, and protein carbonyl in lung tissue were compared between the LPS plus hyperoxia, the LPS only, the hyperoxia only, and the control groups. Results: Morphometric analysis of lung tissues demonstrated that alveolarization was significantly inhibited only in the LPS plus hyperoxia group. IL-6 protein levels and its mRNA expression in the lungs were significantly increased only in the LPS plus hyperoxia group. Neither LPS nor hyperoxia increased IL-6 in the lungs independently. bFGF mRNA expression was significantly decreased in the LPS-treated groups. VEGF protein levels were significantly reduced by hyperoxia, whereas protein carbonyl levels were increased by intra-amniotic LPS or hyperoxia. No additional significant change to VEGF or protein carbonyl levels was produced by intra-amniotic LPS or hyperoxia. There were no significant differences in the mRNA expressions of VEGF, VEGFR-2, and TGF-beta 1. Conclusions: The priming effect of intra-amniotic LPS on hyperoxic lung injury may be associated with IL-6 elevation in the lungs. Copyright (C) 2009 S. Karger AG, Basel
ISSN
1661-7800
Language
English
URI
http://hdl.handle.net/10371/76994
DOI
https://doi.org/10.1159/000263056
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College of Medicine/School of Medicine (의과대학/대학원)Pediatrics (소아과학전공)Journal Papers (저널논문_소아과학전공)
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