Browse

Bacteroides fragilis Enterotoxin Induces Human beta-Defensin-2 Expression in Intestinal Epithelial Cells via a Mitogen-Activated Protein Kinase/I kappa B Kinase/NF-kappa B-Dependent Pathway

Cited 36 time in Web of Science Cited 34 time in Scopus
Authors
Yoon, Young Mee; Lee, Jin Young; Yoo, Doyoung; Sim, Young-Suk; Oh, Yu-Kyoung; Kim, Sunil; Kim, Jung Mogg; Kim, Joo Sung; Kang, Ju Seop; Kim, Young-Jeon
Issue Date
2010-05
Publisher
AMER SOC MICROBIOLOGY
Citation
INFECTION AND IMMUNITY; Vol.78 5; 2024-2033
Abstract
Enterotoxigenic Bacteroides fragilis (ETBF) produces an approximately 20-kDa heat-labile enterotoxin (BFT) that plays an essential role in mucosal inflammation. Although spontaneous disappearance of ETBF infection is common, little information is available on regulated expression of antibacterial factors in response to BFT stimulation. This study investigates the role of BFT in human beta-defensin 2 (hBD-2) induction from intestinal epithelial cells. Stimulation of HT-29 and Caco-2 intestinal epithelial cell lines with BFT resulted in the induction of hBD-2. Activation of a reporter gene for hBD-2 was dependent on the presence of NF-kappa B binding sites. In contrast, suppression of AP-1 did not affect hBD-2 expression in BFT-stimulated cells. Inhibition of p38 mitogen-activated protein kinase (MAPK) using SB203580 and small interfering RNA (siRNA) transfection resulted in a significant reduction in BFT-induced I kappa B kinase (IKK)/NF-kappa B activation and hBD-2 expression. Our results suggest that a pathway including p38 MAPK, IKK, and NF-kappa B activation is required for hBD-2 induction in intestinal epithelial cells exposed to BFT, and may be involved in the host defense following infection with ETBF.
ISSN
0019-9567
Language
English
URI
https://hdl.handle.net/10371/77432
DOI
https://doi.org/10.1128/IAI.00118-10
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse