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CKD-602, a camptothecin derivative, inhibits proliferation and induces apoptosis in glioma cell lines

Cited 16 time in Web of Science Cited 15 time in Scopus
Issue Date
2009-06
Publisher
SPANDIDOS PUBL LTD
Citation
ONCOLOGY REPORTS; Vol.21 6; 1413-1419
Keywords
belotecancamptothecin analogueCKD-602topoisomerase 1 inhibitormalignant glioma
Abstract
CKD-602 7-[2-(N-isopropylamino)ethyl]-(20S)camptothecin, belotecan) is a synthetic water-soluble camptothecin derivative and topoisornerase inhibitor that has been shown to have clinical anticancer effect against ovarian and lung cancer. We studied its anticancer effects oil four human glioma cell lines, U87 MG, U343 MG, U251 MG and LN229. Cell viability was quantified by a modified 2-(2-methoxy-4-methoxy)-3-(4-nitropheyl)-5-(2,4-disulfophellyl) -2H-tetrazolium, monosodium salt and significant time- and dose-dependent cytotoxicity was observed in all cell lines. Susceptibility to CKD-602 at 48 h after treatment varied among the four cell lines and their IC(50) value was as follows: 9.07 nM (95% CI 0.18-37.42) for LN229, 14.57 nM (95% Cl 0.86-47.33) for U251 MG, 29.13 nM (95% CI 0.35-101.23) for U343 MG, and 84.66 nM (95% CI 34.63-148.25) for U87 MG. CKD-602 induced cell cycle arrest at G2 phase and produced antiproliferative activity and apoptosis in all cell lines. Thus, CKD-602 showed a significant anticancer effect on glioma cells in vitro and is a promising candidate for further studies on malignant gliomas.
ISSN
1021-335X
Language
English
URI
https://hdl.handle.net/10371/77624
DOI
https://doi.org/10.3892/or_00000369
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College of Medicine/School of Medicine (의과대학/대학원)Neurosurgery (신경외과학전공)Journal Papers (저널논문_신경외과학전공)
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