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Protein Expression Profiling of Primary Mammary Epithelial Cells Derived from MMTV-neu Mice Revealed that HER2/NEU-Driven Changes in Protein Expression Are Functionally Clustered

Cited 3 time in Web of Science Cited 3 time in Scopus
Authors
Park, Sungwoo; Lee, Kyung-min; Ju, Ji-hyun; Kim, Jaeyoon; Lee, Taehoon; Shin, Incheol; Noh, Dong-Young
Issue Date
2010-01
Publisher
JOHN WILEY & SONS INC
Citation
IUBMB LIFE; Vol.62 1; 41-50
Keywords
proteonomicssignal transductionHER2/NEU
Abstract
MMTV-neu transgenic mice overexpressing NEU in their mammary glands develop tumor after 6 months of age. To find a novel protein biomarker using this mouse model, we identified and characterized the proteins that were differently expressed between primary mammary epithelial cells from 2 months old MMTV-neu heterozygote mice and wild type (WT) littermates using two-dimensional digest (ChemDigest (TM)/Trypsin)-LC-MS/MS. The differentially expressed proteins were selected and analyzed using DAVID Bioinformatics resource. The proteins involved in anti-apoptosis, purine metabolism, ribosome and proteasome functions were upregulated, whereas cell adhesion-related proteins were downregulated in PMECs from MMTV-neu mice when compared with WT PMECs. The results indicate that several functional units are coregulated by HER2/NEU. We hypothesize that these changes in the cellular proteome may be responsible for early onset of HER2/NEU-driven tumorigenesis. (C) 2009 IUBMB IUBMB Life, 62(1): 41-50, 2010
ISSN
1521-6543
Language
English
URI
http://hdl.handle.net/10371/77942
DOI
https://doi.org/10.1002/iub.276
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College of Medicine/School of Medicine (의과대학/대학원)Surgery (외과학전공)Journal Papers (저널논문_외과학전공)
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