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Gold nanoparticles attenuate LPS-induced NO production through the inhibition of NF-kappa B and IFN-beta/STAT1 pathways in RAW264.7 cells

Cited 73 time in Web of Science Cited 79 time in Scopus
Authors
Ma, Ji Su; Kim, Wan Jae; Kim, Jae Jin; Kim, Tack Joong; Song, Min Dong; Kim, Dong Woo; Lee, Kwang Ho; Moon, Won Kook; Kang, Hyun; Ye, Sang Kyu
Issue Date
2010-11-01
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
NITRIC OXIDE-BIOLOGY AND CHEMISTRY; Vol.23 3; 214-219
Keywords
Gold nanoparticlesInterferon-beta (IFN-beta)Inducible nitric synthase (iNOS)Nitric oxide (NO)Signal transducer and activator of transcription 1 (STAT1)Lipopolysaccharide (LPS)
Abstract
Macrophage-derived nitric oxide (NO) plays an important role in protection against microbial infection in immune responses. Overproduction of NO by inducible nitric synthase (iNOS) is known to be closely correlated with the pathology of a variety of diseases and inflammations. In this study, we investigated the inhibitory effect of polyethylene glycol coated gold nanoparticles (GNP) on NO production and its molecular mechanism in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. It was found that GNP inhibited LPS-induced NO production and iNOS expression in RAW264.7 cells. Furthermore, GNP suppressed LPS-induced activation of NF-kappa B through the inhibition of Akt activity. GNP also inhibited LPS-induced phosphorylation of signal transducer and activator of transcription 1 (STAT1) via down-regulation of interferon-beta (IFN-beta) expression. Our results suggest that GNP inhibits NO production and iNOS expression through blocking the activation of NF-kappa B and STAT1 in LPS-stimulated RAW264.7 cells. (C) 2010 Elsevier Inc. All rights reserved.
ISSN
1089-8603
Language
English
URI
https://hdl.handle.net/10371/78067
DOI
https://doi.org/10.1016/j.niox.2010.06.005
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College of Medicine/School of Medicine (의과대학/대학원)Pharmacology (약리학전공)Journal Papers (저널논문_약리학전공)
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