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Bioimaging of Nucleolin Aptamer-Containing 5-(N-benzylcarboxyamide)-2 `-deoxyuridine More Capable of Specific Binding to Targets in Cancer Cells

Cited 38 time in Web of Science Cited 39 time in Scopus
Authors
Lee, Kyue Yim; Kang, Hyungu; Ryu, Sung Ho; Lee, Dong Soo; Kim, Soonhag; Lee, Jung Hwan
Issue Date
2010
Publisher
HINDAWI PUBLISHING CORPORATION
Citation
JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY; Vol. ; -
Abstract
Chemically modified nucleotides have been developed and applied into SELEX procedure to find a novel type of aptamers to fit with targets of interest. In this study, we directly performed chemical modification of 5-(N-benzylcarboxyamide)-2`-deoxyuridine (called 5-BzdU) in the AS1411 aptamer, which binds to the nucleolin protein expressed in cancer cells. Forty-seven compounds of AS1411-containing Cy3-labeled 5-BzdU (called Cy3-(5-BzdU)-modified-AS1411) were synthesized by randomly substituting thymidines one to twelve in AS1411 with Cy3-labeled 5-BzdU. Both statistically quantified fluorescence measurements and confocal imaging analysis demonstrated at least three potential compounds of interest: number 12, 29 and 41 that significantly increased the targeting affinity to cancer cells but no significant activity from normal healthy cells. These results suggest that the position and number of substituents in AS1411 are critical parameters to improve the aptamer function. In this study, we demonstrated that chemical modification of the existing aptamers enhanced the binding and targeting affinity to targets of interest without additional SELEX procedures.
ISSN
1110-7243
Language
English
URI
http://hdl.handle.net/10371/78294
DOI
https://doi.org/10.1155/2010/168306
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College of Medicine/School of Medicine (의과대학/대학원)Nuclear Medicine (핵의학전공)Journal Papers (저널논문_핵의학전공)
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