Browse

In Vivo Imaging of Sentinel Nodes Using Fluorescent Silica Nanoparticles in Living Mice

Cited 22 time in Web of Science Cited 27 time in Scopus
Issue Date
2010-04
Publisher
SPRINGER
Citation
MOLECULAR IMAGING AND BIOLOGY; Vol.12 2; 155-162
Keywords
Silica nanoparticlesSentinel nodeOptical imaging
Abstract
We examine the feasibility of fluorescent imaging system for sentinel lymph node detection by using functionalized silica nanoparticles. We developed a functionalized RITC-SiO(2) nanoparticles containing fluorescent dye, C(28)H(31)N(2)O(3)Cl (rhodamine B isothiocyanate) inside, and subsequently synthesized (68)Ga-NOTA-RITC-SiO(2) nanoparticles. At 5 min after RITC-doped silica nanoparticles injection, fluorescent signals were shown in both right axillary lymph node (ALN) and injection site of living mice. Fluorescent signals were also observed at these locations in a biodistribution study. In addition, fluorescence was detected in frozen ALN sections microscopically. The percentages of doses injected per gram of tissue of axillary and brachial lymph nodes near footpad treated with (68)Ga-NOTA-RITC-SiO(2) nanoparticles were 308.3 +/- 3.4 and 41.5 +/- 6.1, respectively. Little (68)Ga radioactivity was found in other organs. Our data provide strong evidence that functionalized silica nanoparticles has a promising potential as organic lymphatic tracer in biomedical imaging such as pre- and intraoperative surgical guidance.
ISSN
1536-1632
Language
English
URI
https://hdl.handle.net/10371/78303
DOI
https://doi.org/10.1007/s11307-009-0262-8
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Nuclear Medicine (핵의학전공)Journal Papers (저널논문_핵의학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse