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In Vivo Bioluminescent Imaging of Rat Mesenchymal Stem Cells in a Mouse Model of Hindlimb Ischemia

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Authors
Chung, Hye Kyung; Chung, June-Key; Kang, Joo Hyun; Kim, Seunghoo; Lee, Dong Soo; Kim, Hyun Joo
Issue Date
2009-06
Publisher
KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC
Citation
TISSUE ENGINEERING AND REGENERATIVE MEDICINE; Vol.6, Issues 4-11; 668-674
Keywords
Stem cellsreporter gene imagingfirefly luciferasehindlimb ischemia
Abstract
We developed a luciferase gene imaging system Using a retrovirus for monitoring mesenchymal stem cell distribution. Retrovirus expressing firefly luciferase was infected into rat mesenchymal stein cells(rMSCs). rMSC-luc cells were excellent correlations between in vitro and in vivo bioluminescent activities and cell numbers. After systemic injections of cells in mice, luminescence activity was observed until six days, but was not detected in ischemic regions. After local injection with scaffold, luminescence activities were detected in injection sites over 3 weeks. This system Could be a useful means for in vivo real time imaging of stem cell distributions, viability, and trafficking.
ISSN
1738-2696
Language
English
URI
https://hdl.handle.net/10371/78587
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College of Medicine/School of Medicine (의과대학/대학원)Nuclear Medicine (핵의학전공)Journal Papers (저널논문_핵의학전공)
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