Publications
Detailed Information
In Vivo Bioluminescent Imaging of Rat Mesenchymal Stem Cells in a Mouse Model of Hindlimb Ischemia
Cited 0 time in
Web of Science
Cited 0 time in Scopus
- Authors
- Issue Date
- 2009-06
- Citation
- TISSUE ENGINEERING AND REGENERATIVE MEDICINE; Vol.6, Issues 4-11; 668-674
- Keywords
- Stem cells ; reporter gene imaging ; firefly luciferase ; hindlimb ischemia
- Abstract
- We developed a luciferase gene imaging system Using a retrovirus for monitoring mesenchymal stem cell distribution. Retrovirus expressing firefly luciferase was infected into rat mesenchymal stein cells(rMSCs). rMSC-luc cells were excellent correlations between in vitro and in vivo bioluminescent activities and cell numbers. After systemic injections of cells in mice, luminescence activity was observed until six days, but was not detected in ischemic regions. After local injection with scaffold, luminescence activities were detected in injection sites over 3 weeks. This system Could be a useful means for in vivo real time imaging of stem cell distributions, viability, and trafficking.
- ISSN
- 1738-2696
- Language
- English
- Files in This Item:
- There are no files associated with this item.
- Appears in Collections:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.