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In vivo alternative testing with zebrafish in ecotoxicology
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- Authors
- Issue Date
- 2008
- Citation
- J Vet Sci 2008, 9, 351-357
- Keywords
- aryl hydrocarbon receptor ; enhanced green fluorescent ; zebrafish
- Abstract
- Although rodents have previously been used in
ecotoxicological studies, they are expensive, time-consuming,
and are limited by strict legal restrictions. The present
study used a zebrafish (Danio rerio) model and generated
data that was useful for extrapolating toxicant effects in
this system to that of humans. Here we treated embryos of
the naive-type as well as a transiently transfected
zebrafish liver cell line carrying a plasmid (phAhREEGFP),
for comparing toxicity levels with the well-known
aryl hydrocarbon receptor (AhR)-binding toxicants:
3,3,4,4,5-pentachlorobiphenyl (PCB126), 2,3,7,8-tetrachlorodibenzo-
p-dioxin, and 3-methylcholanthrene. These
toxicants induced a concentration-dependent increase in
morphological disruption, indicating toxicity at early
life-stages. The transient transgenic zebrafish liver cell
line was sensitive enough to these toxicants to express the
CYP1A1 regulated enhanced green fluorescent protein.
The findings of this study demonstrated that the zebrafish
in vivo model might allow for extremely rapid and
reproducible toxicological profiling of early life-stage
embryo development. We have also shown that the
transient transgenic zebrafish liver cell line can be used
for research on AhR mechanism studies.
- ISSN
- 1229-845X
- Language
- English
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