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Modulation of Cultured Neural Networks Using Neurotrophin Release

Cited 13 time in Web of Science Cited 17 time in Scopus
Authors
Jun, Sang Beom; Hynd, Matthew R.; Dowell-Mesfin, Natalie M.; Al-Kofahi, Yousef; Roysam, Badrinath; Shain, William; Kim, Sung June
Issue Date
2008-05
Publisher
Institute of Physics
Citation
J. Neural. Eng. 5 203-213
Abstract
Polyacrylamide and poly(ethylene glycol) diacrylate hydrogels were synthesized and
characterized for use as drug release and substrates for neuron cell culture. Protein release
kinetics was determined by incorporating bovine serum albumin (BSA) into hydrogels during
polymerization. To determine if hydrogel incorporation and release affect bioactivity, alkaline
phosphatase was incorporated into hydrogels and a released enzyme activity determined using
the fluorescence-based ELF-97 assay. Hydrogels were then used to deliver a brain-derived
neurotrophic factor (BDNF) from hydrogels polymerized over planar microelectrode arrays
(MEAs). Primary hippocampal neurons were cultured on both control and
neurotrophin-containing hydrogel-coated MEAs. The effect of released BDNF on neurite
length and process arborization was investigated using automated image analysis. An
increased spontaneous activity as a response to the released BDNF was recorded from the
neurons cultured on the top of hydrogel layers. These results demonstrate that proteins of
biological interest can be incorporated into hydrogels to modulate development and function of
cultured neural networks. These results also set the stage for development of hydrogel-coated
neural prosthetic devices for local delivery of various biologically active molecules.
ISSN
1741-2560 (print)
1741-2552 (online)
Language
English
URI
https://hdl.handle.net/10371/7921
DOI
https://doi.org/10.1088/1741-2560/5/2/011
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Electrical and Computer Engineering (전기·정보공학부)Journal Papers (저널논문_전기·정보공학부)
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