Patterned cell culture inside microfluidic devices
- Issue Date
- Royal Society of Chemistry
- Lab Chip, 2004, 5, 102-107
- This paper describes a simple plasma-based dry etching method that enables patterned cell culture
inside microfluidic devices by allowing patterning, fluidic bonding and sterilization steps to be
carried out in a single step. This plasma-based dry etching method was used to pattern celladhesive
and non-adhesive areas on the glass and polystyrene substrates. The patterned substrate
was used for selective attachment and growth of human umbilical vein endothelial cells, MDAMB-
231 human breast cancer cells, NIH 3T3 mouse fibroblasts, and primary rat cortical neurons.
Finally, we have successfully combined the dry-patterned substrate with a microfluidic device.
Patterned primary rat neurons were maintained for up to 6 days inside the microfluidic devices
and the neurons somas and processes were confined to the cell-adhesive region. The method
developed in this work offers a convenient way of micropatterning biomaterials for selective
attachment of cells on the substrates, and enables culturing of patterned cells inside microfluidic
devices for a number of biological research applications where cells need to be exposed to wellcontrolled