Neutrophil Migration in Opposing Chemoattractant Gradients Using Microfluidic Chemotaxis Devices
- Lin, Francis; Nguyen, Connie Minh-Canh; Wang, Shur-Jen; Saadi, Wajeeh; Gross, Steven P.; Jeon, Noo Li
- Issue Date
- Springer Verlag
- Ann. Biomed. Eng. 33:475-482, 2005
- Neutrophils migrating in tissue respond to complex overlapping signals generated by a variety of chemotactic factors (CFs). Previous studies suggested a hierarchy between bacteriaderived CFs and host-derived CFs but could not differentiate neutrophil
response to potentially equal host-derived CFs (IL-8 and LTB4). This paper reports neutrophil migration in conflicting gradients
of IL-8 and LTB4 using a microfluidic chemotaxis device that can generate stable and well-defined gradients. We quantitatively
characterized the movement of cells from time-lapse images. Neutrophils migrate more efficiently toward single IL-8 gradients than single LTB4 gradients as measured by the effective chemotactic index (ECI). In opposing gradients of IL-8 and LTB4,
neutrophils show obvious chemotaxis toward a distant gradient, consistent with previous reports. When an opposing gradient of
LTB4 is present, neutrophils show less effective chemotaxis toward IL-8 than when they are in a gradient of IL-8 alone. In
contrast, the chemotactic response of neutrophils to LTB4 is not reduced in opposing gradients as compared to that in a single LTB4 gradient. These results indicate that the presence of one host-derived CF modifies the response of neutrophils to a second CF suggesting a subtle hierarchy between them.
- 0090-6964 (print)
- Files in This Item: There are no files associated with this item.