S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 23 No.1 (1982)
약물반응의 진단에 대한 연층백혈구 응집검사법의 응용에 관한 연구
Application of the Buffy Coat Leucocyte Aggregation (BCLA) Test in Diagnosis of Drug Reaction
- 김원석; 장우현; 조한익; 이유신; 김홍식; 문기찬; 신용우; 이승훈
- Issue Date
- 서울대학교 의과대학
- Seoul J Med, Vol.23 No.1, pp. 97-101
- Buffy coat leucocyte aggregation (BCLA) in the
presence of a specific antigen was suggested a simple.
sensitive and highly reproducible in vitro method to
test the cell-mediated immunity to certain antigens.
The cell-mediated immune response is known to be
responsible for certain exanthematous drug reaction
and contact hypersensitivity to chemicals.
The present study was undertaken to evaluate the
BCLA test as a possible in vitro diagnostic aid to
detect the drug reactions and contact hypersensitivity
to certain chemicals. Twenty-two patients with
ampicillin drug eruption, contact dermatitis to nickel
sulfate, paraphenylenediamine, potassium dichromate
and mercuric chloride and 68 control subjects entered
this study. The method of the BCLA test was same as described by the authors elsewhere (]979). At
various concentrations of cah antigen ranging from
2. 5 X 10 to 2.5 x 10-' mg/ml, none of the patients
and the control subjects showed the BCLA.
The authors concluded that in these antigen systems
the BCLA test could not be a diagnostic method.
The possible mechanism of this negative results