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Rho-associated kinase (ROCK) inhibition reverses low cell activity on hydrophobic surfaces

Cited 18 time in Web of Science Cited 18 time in Scopus
Authors
Tian, Yu Shun; Kim, Hyun Jung; Kim, Hyun-Man
Issue Date
2009-08-28
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.386, No.3, pp.499-503
Keywords
HydrophobicityRho-associated kinaseY-27632OsteoblastCell cycleMigrationAdhesionTissue engineeringBiomaterial
Abstract
Hydrophobic polymers do not offer an adequate scaffold surface for cells to attach, migrate, proliferate, and differentiate. Thus, hydrophobic scaffolds for tissue engineering have traditionally been physicochemically modified to enhance cellular activity. However, modifying the surface by chemical or physical treatment requires Supplementary engineering procedures. In the present study, regulation of a cell signal transduction pathway reversed the low Cellular activity on a hydrophobic surface without Surface modification. inhibition of Rho-associated kinase (ROCK) by Y-27632 markedly enhanced adhesion, Migration, and proliferation of osteoblastic cells cultured on a hydrophobic polystyrene Surface. ROCK inhibition regulated cell-cycle-related molecules on the hydrophobic Surface. This inhibition also decreased expression of the inhibitors of cyclin-dependent kinases such as p21(cip1) and p27(kip1) and increased expression of cyclin A and D. These results indicate that defective cellular activity on the hydrophobic Surface can be reversed by the control of a cell signal transduction pathway Without physicochemical surface modification. (C) 2009 Elsevier Inc. All rights reserved.
ISSN
0006-291X
Language
English
URI
https://hdl.handle.net/10371/80369
DOI
https://doi.org/10.1016/j.bbrc.2009.06.087
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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