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A Biologically Active Sequence of the Laminin alpha 2 Large Globular 1 Domain Promotes Cell Adhesion through Syndecan-1 by Inducing Phosphorylation and Membrane Localization of Protein Kinase C delta

Cited 17 time in Web of Science Cited 17 time in Scopus
Authors
Jung, Sung Youn; Kim, Jin-Man; Jang, Da Hyun; Kang, Hyun Ki; Min, Byung-Moo
Issue Date
2009-11-13
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY, Vol.284, No.46, pp.31764-31775
Abstract
Laminin-2 promotes basement membrane assembly and peripheral myelinogenesis; however, a receptor-binding motif within laminin-2 and the downstream signaling pathways for motif-mediated cell adhesion have not been fully established. The human laminin-2 alpha 2 chain cDNAs cloned from human keratinocytes and fibroblasts correspond to the laminin alpha 2 chain variant sequence from the human brain. Individually expressed recombinant large globular (LG) 1 protein promotes cell adhesion and has heparin binding activities. Studies with synthetic peptides delineate the DLTIDDSYWYRI motif (Ln2-P3) within the LG1 as a major site for both heparin and cell binding. Cell adhesion to LG1 and Ln2-P3 is inhibited by treatment of heparitinase I and chondroitinase ABC. Syndecan-1 from PC12 cells binds to LG1 and Ln2-P3 and colocalizes with both molecules. Suppression of syndecan-1 with RNA interference inhibits cell adhesion to LG1 and Ln2-P3. The binding of syndecan-1 with LG1 and Ln2-P3 induces the recruitment of protein kinase C delta (PKC delta) into the membrane and stimulates its tyrosine phosphorylation. A decrease in PKC delta activity significantly reduces cell adhesion to LG1 and Ln2-P3. Taken together, these results indicate that the Ln2-P3 motif and LG1 domain, containing the motif, within the human laminin-2 alpha 2 chain are major ligands for syndecan-1, which mediates cell adhesion through the PKC delta signaling pathway.
ISSN
0021-9258
Language
English
URI
http://hdl.handle.net/10371/80384
DOI
https://doi.org/10.1074/jbc.M109.038547
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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