S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Effect of hydroxyapatite-coated nanofibrous membrane on the responses of human periodontal ligament fibroblast
- Issue Date
- JOURNAL OF THE CERAMIC SOCIETY OF JAPAN, Vol.116, No.1349, pp.31-35
- biomaterial ; hydroxyapatite ; membrane ; periodontal ligament fibroblast ; periodontal tissue regeneration
- In this study, hydroxyapatite-coated biopolymer nanofibrous membrane was synthesized by mineralizing the electrospun polycaprolactone nanofiber. The effect of the hydroxyapatite-coated nanofibrous membrane was mainly investigated on the proliferation and differentiation of human periodontal ligament fibroblast, which is necessary for periodontal tissue regeneration. Scanning electron microscopy revealed favorable cell attachment and spreading appearance on 1 d and MTS assay showed increased cell proliferation on the hydroxyapatite-coated nanofiber membrane during 14 d. From 7 to 14 d, alkaline phosphatase activity of the hydroxyapatite-coated nanofiber was significantly increased when compared to that of control group. Hydroxyapatite-coated nanofibrous membrane showed prominent mineral formation on day 14. As a result, it was clarified that the developed hydroxyapatite-coated nanofibrous membrane had favorable effects on the proliferation and differentiation of human periodontal ligament fibroblast and might be a good candidate material for periodontal tissue regeneration.